Suppression of neutral cholesterol ester hydrolase activity by antisense DNA of hormone-sensitive lipase

Biochemical and Biophysical Research Communications
Jun-ichi OsugaN Yamada

Abstract

In order to investigate the role of the hormone-sensitive lipase (HSL) gene in the activity of neutral cholesterol ester hydrolase (NCEH) from a molecular perspective, Chinese hamster ovary (CHO) cells were transfected with rat antisense hormone-sensitive lipase cDNA, and three different cell lines, designated as anti-HSL, were established. NCEH activity in anti-HSL cells was reduced to approximately 50% of that in control CHO cells. The concentration of cellular esterified cholesterol increased and the concentration of free cholesterol decreased in the anti-HSL cell lines. These results suggest that the HSL gene has a function of NCEH as well.

References

Sep 1, 1991·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·I InoueN Yamada
Apr 9, 1990·Biochimica Et Biophysica Acta·S J Yeaman
Sep 14, 1995·Biochemical and Biophysical Research Communications·J OsugaS Ishibashi
Feb 1, 1994·Arteriosclerosis and Thrombosis : a Journal of Vascular Biology·M KawamuraK Kozaki

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Citations

Jun 8, 2000·Annals of the New York Academy of Sciences·C LondosC M Rondinone
Jan 19, 2011·Gene·Antonis D LampidonisDimitrios J Stravopodis
Jun 22, 2000·Biology of Reproduction·B D Murphy
Oct 1, 2019·Therapeutic Advances in Endocrinology and Metabolism·Yu-Long LanBo Zhang

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