Suppression of peptide ion dissociation under electron capture: role of backbone amide hydrogen

Rapid Communications in Mass Spectrometry : RCM
P-S Joyce WongT-W Dominic Chan

Abstract

The electron capture dissociation (ECD) of proteins/peptides is affected by the nature and sequence of amino acid residues. Electron capture/transfer with no dissociation is an intriguing phenomenon that has occasionally been observed. We have previously identified that diarginated peptides enriched with glutamic acid residues were found to show suppression of backbone fragmentation. In this paper, we report the effect of geometrical parameters of a peptide, including chain length, conformation and amide hydrogen, on the suppression of ECD fragmentation using synthetic model peptides. Glycine containing model polypeptides were used to probe the mechanism. Molecular-mechanics was used to obtain the conformation of the precursor ions. The ECD experiments were performed on a Bruker APEX III 4.7 T Fourier transform ion cyclotron resonance (FTICR) mass spectrometer. Significant decreases in the intensities of the fragment ions were observed for the 23-mer polypeptide with only one E residue. This implied that the E:R ratio was no longer the sole determining factor for the occurrence of suppression effects. Results of conformational searches showed that there was a hydrogen-bonding 'ladder' formed in the 23-mer polypeptide, which was...Continue Reading

References

Jan 15, 1999·Journal of the American Society for Mass Spectrometry·K W Fong, T W Chan
May 30, 2002·Journal of the American Chemical Society·Kathrin BreukerFred W McLafferty
Dec 18, 2002·Journal of the American Society for Mass Spectrometry·T W Dominic Chan, W H Herman Ip
Dec 14, 2004·Journal of the American Society for Mass Spectrometry·Romulus MihalcaRon M A Heeren
Apr 8, 2006·The Journal of Physical Chemistry. B·Christopher F RodriquezK W Michael Siu
Aug 1, 2006·Journal of the American Society for Mass Spectrometry·Marina A BelyayevPeter B O'Connor
Aug 31, 2006·Rapid Communications in Mass Spectrometry : RCM·Asimo KarnezisW David McFadyen
Nov 23, 2006·Journal of the American Society for Mass Spectrometry·Jace W JonesFrantisek Turecek
Oct 24, 2007·Journal of the American Society for Mass Spectrometry·Julia Chamot-RookeFrantisek Turecek
Feb 27, 2008·Angewandte Chemie·Yi He, James P Reilly
Mar 20, 2009·Journal of the American Society for Mass Spectrometry·Hisham Ben HamidaneYury O Tsybin
Apr 1, 2009·Journal of the American Chemical Society·Chang Ho SohnJ L Beauchamp
May 5, 2009·Journal of the American Society for Mass Spectrometry·David M Crizer, Scott A McLuckey
Nov 26, 2009·Journal of the American Society for Mass Spectrometry·Andrew W JonesHelen J Cooper
May 4, 2010·Journal of the American Society for Mass Spectrometry·Wai Yi Kelly Chan, Tak Wah Dominic Chan

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Citations

Feb 9, 2017·Journal of Mass Spectrometry : JMS·Juliette J Commodore, Carolyn J Cassady

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