Suppression of Plant Immune Responses by the Pseudomonas savastanoi pv. savastanoi NCPPB 3335 Type III Effector Tyrosine Phosphatases HopAO1 and HopAO2

Frontiers in Plant Science
María Pilar Castañeda-OjedaEmilia López-Solanilla

Abstract

The effector repertoire of the olive pathogen P. savastanoi pv. savastanoi NCPPB 3335 includes two members of the HopAO effector family, one of the most diverse T3E families of the P. syringae complex. The study described here explores the phylogeny of these dissimilar members, HopAO1 and HopAO2, among the complex and reveals their activities as immune defense suppressors. Although HopAO1 is predominantly encoded by phylogroup 3 strains isolated from woody organs of woody hosts, both HopAO1 and HopAO2 are phylogenetically clustered according to the woody/herbaceous nature of their host of isolation, suggesting host specialization of the HopAO family across the P. syringae complex. HopAO1 and HopAO2 translocate into plant cells and show hrpL-dependent expression, which allows their classification as actively deployed type III effectors. Our data also show that HopAO1 and HopAO2 possess phosphatase activity, a hallmark of the members of this family. Both of them exert an inhibitory effect on early plant defense responses, such as ROS production and callose deposition, and are able to suppress ETI responses induced by the effectorless polymutant of P. syringae pv. tomato DC3000 (DC3000D28E) in Nicotiana. Moreover, we demonstrate t...Continue Reading

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Citations

Aug 8, 2021·Microorganisms·Alba Moreno-PérezLuis Rodríguez-Moreno
Sep 28, 2021·Molecular Plant Pathology·Arya Bagus Boedi IswantoSang Hee Kim

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Datasets Mentioned

BETA
GG774693.1

Methods Mentioned

BETA
dot-blot
PCR
electrophoresis
PCRs
protein
phosphatase assay

Software Mentioned

Geneious
Visilog
Roche LightCycler 480
MEGA7

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