Suppression of PmRab7 by dsRNA inhibits WSSV or YHV infection in shrimp.

Marine Biotechnology
Chalermporn OngvarrasoponeSakol Panyim

Abstract

Viral entry into host cells requires endocytosis machineries of the host for viral replication. PmRab7, a Penaeus monodon small GTPase protein, was investigated for its function in vesicular transport during viral infection. The double-stranded RNA of Rab7 was injected into a juvenile shrimp before challenging with white spot syndrome virus (WSSV) or yellow head virus (YHV). PmRab7 mRNA was specifically decreased at 48 h after dsRNA-Rab7 injection. Silencing of PmRab7 dramatically inhibited WSSV-VP28 mRNA and protein expression. Unexpectedly, the silencing of PmRab7 also inhibited YHV replication in the YHV-infected shrimp. These results suggested that PmRab7 is a common cellular factor required for WSSV or YHV replication in shrimp. Because PmRab7 should function in the endosomal trafficking pathway, its silencing prevents successful viral trafficking necessary for replication. Silencing of PmRab7 could be a novel approach to prevent both DNA virus (WSSV) and RNA virus (YHV) infection of shrimp.

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Citations

Apr 16, 2010·Marine Biotechnology·T W Flegel, Kallaya Sritunyalucksana
Apr 17, 2010·Marine Biotechnology·Ikuo HironoTakashi Aoki
Dec 18, 2013·Developmental and Comparative Immunology·Thapanan JatuyospornKuakarun Krusong
Feb 2, 2016·Developmental and Comparative Immunology·Phattarunda JareeKunlaya Somboonwiwat
Jan 16, 2016·Journal of Invertebrate Pathology·Chaweewan ChimwaiPongsopee Attasart
Jan 22, 2013·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Feb 19, 2013·Fish & Shellfish Immunology·Kallaya SritunyalucksanaJiraporn Srisala
Jan 2, 2013·Fish & Shellfish Immunology·Paula C LimaMathew Cook
Oct 2, 2012·Fish & Shellfish Immunology·Kallaya SritunyalucksanaJiraporn Srisala
May 12, 2012·Journal of Biotechnology·Gatesara TheerawanitchpanVanvimon Saksmerprome
Aug 2, 2011·Developmental and Comparative Immunology·Qian RenJin-Xing Wang
Jul 13, 2011·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Nov 9, 2010·Protein Expression and Purification·Natapong JupatanakulKallaya Sritunyalucksana
Jul 14, 2010·Antiviral Research·Chalermporn OngvarrasoponeSakol Panyim
Feb 19, 2009·Fish & Shellfish Immunology·Haipeng LiuPikul Jiravanichpaisal
Aug 3, 2013·Marine Biotechnology·Sajad AhangerRakesh K Mishra
Jul 5, 2013·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Oct 1, 2013·Developmental and Comparative Immunology·Wanchart YingvilasprasertAnchalee Tassanakajon
Mar 19, 2014·Journal of Biotechnology·Poohrawind SanittSakol Panyim
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Jul 27, 2015·Molecular Immunology·Yaodong HeXiaobo Zhang
Jun 12, 2013·Fish & Shellfish Immunology·Zih-Jhan HuangLi-Li Chen
Feb 11, 2015·Developmental and Comparative Immunology·Jie LiXu-Sheng Liu
Mar 1, 2015·Fish & Shellfish Immunology·Amnat PhetrungnaphaChalermporn Ongvarrasopone
Jun 3, 2014·Antiviral Research·Pei-Hui WangJian-Guo He
Aug 12, 2014·Developmental and Comparative Immunology·Dandan XuTianzhi Huang
Apr 27, 2016·Molecular Biology Reports·Wenting DaiQibin Yang

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