Abstract
To determine the effect(s) of reactive sulfonamide metabolites on antibody production by human lymphocytes. Human peripheral blood cells (PBMCs) were isolated from control volunteers and incubated with the hydroxylamine of sulfamethoxazole (SMX H/A), a reactive metabolite of the most commonly used sulfonamide, in increasing concentrations. PBMCs were then stimulated to produce antibody with pokeweed mitogen. After incubation for 8 days, concentrations of IgG and IgM were determined in supernatant using an ELISA assay. Production of both IgG and IgM was significantly suppressed by sub-lethal concentrations of SMX H/A in a concentration-dependent fashion (p < 0.05). Suppression was more marked for IgM production (maximal decline to 80% of baseline antibody production) than for IgG production (maximal decline to 57% of baseline antibody production). No suppression was seen when cells were incubated with sulfamethoxazole in concentrations up to 400 microM. This suppression was not related to changes in cell viability; at a concentration of 25 microM of SMX H/A, IgM and IgG concentration were reduced by 47 +/- 8.7% and 73 +/- 7.2%, while cell viability (percentage of live cells) was 93 +/- 5%. Suppression was time-dependent, increas...Continue Reading
References
Feb 1, 1978·The Journal of Pediatrics·W T HughesS K Sanyal
May 1, 1992·Canadian Journal of Physiology and Pharmacology·M J RiederI A Bird
Oct 1, 1992·International Journal of Immunopharmacology·M J RiederW Y Almawi
Feb 23, 1991·Lancet·J Ruskin, M LaRiviere
Feb 15, 1991·Biochemical Pharmacology·J S LeederH M Dosch
Mar 1, 1987·Biochemical Pharmacology·B K ParkN R Kitteringham
Dec 1, 1989·Current Opinion in Immunology·L E Samelson
Feb 15, 1989·Annals of Internal Medicine·M J RiederS P Spielberg
Jun 25, 1987·The New England Journal of Medicine·W T HughesL Lott
Aug 1, 1986·Annals of Internal Medicine·N H ShearW Kalow
Nov 1, 1985·Canadian Journal of Physiology and Pharmacology·N H Shear, S P Spielberg
Oct 1, 1984·The Journal of Allergy and Clinical Immunology·A P Kaplan
Sep 15, 1980·Experientia·K HoshiT Ogita
Jan 6, 1995·Toxicology·M J RiederI A Bird
Feb 1, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·M J RiederG A Dekaban
Citations
Jan 1, 2001·Indian Journal of Clinical Biochemistry : IJCB·G R DuddukuriR R Athota
Apr 16, 2010·Environmental Technology·Y LesterH Mamane
Aug 19, 2005·Life Sciences·D Siva Sundara KumarKevin D Hyde
Aug 2, 2001·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A HessM J Rieder
Oct 3, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·D A HessM J Rieder
May 11, 2018·Virology Journal·Kemi AdeyanjuGregory A Dekaban
Apr 2, 2003·Environmental Science & Technology·Marc M HuberUrs von Gunten