Suppression of proline requirement of proA and proAB deletion mutants in Salmonella typhimurium by mutation to arginine requirement.

Journal of Bacteriology
T T Kuo, B A Stocker

Abstract

Eleven variants able to grow without proline (provided arginine was absent) were obtained by spontaneous mutation from Salmonella typhimurium LT7 proA and proAB deletion mutants. Suppression resulted from mutation at argG, which specifies N(alpha)-acetylornithine delta-transaminase. In the absence of exogenous arginine, deficiency of this enzyme would cause derepression of the arginine pathway and accumulation of N-acetylglutamic gamma-semialdehyde. N-acetylglutamic gamma-semialdehyde, if deacetylated, would produce glutamic gamma-semialdehyde, the proline precursor whose synthesis from glutamate is blocked in proA and proAB mutants. All of the mutants grew only slowly (some very slowly) if not supplied with arginine. Sonic-treated preparations of eight mutants had no measurable acetylornithine delta-transaminase activity, but those of the three mutants least dependent on arginine had 0.11, 0.28, and 1.48 of wild-type activity; presumably, their enzymes have low specific activity, at least in vivo. Phage P22 cotransduced argG and strA. Genetic analysis showed that the minor degree of arginine dependence of the mutant with greater than wild-type in vitro enzyme activity was a characteristic of its argG allele, not the result of ...Continue Reading

References

Dec 1, 1967·Bacteriological Reviews·A L Taylor, C D Trotter
Dec 1, 1967·Bacteriological Reviews·K E Sanderson
Mar 1, 1968·Journal of Bacteriology·H Itikawa, M Demerec
Jan 1, 1966·Annual Review of Microbiology·L Gorini, J R Beckwith
Oct 1, 1965·Virology·H O Smith, M Levine
Feb 1, 1967·Virology·H O Smith, M Levine
Jan 1, 1961·Cold Spring Harbor Symposia on Quantitative Biology·L GORINIM BURGER
Jan 1, 1961·Cold Spring Harbor Symposia on Quantitative Biology·H J VOGEL
Mar 1, 1963·Archives of Biochemistry and Biophysics·S VYAS, W K MAAS
Jan 1, 1961·Cold Spring Harbor Symposia on Quantitative Biology·W K MAAS
Jun 1, 1960·Genetics·T Miyake, M Demerec

❮ Previous
Next ❯

Citations

Jan 1, 1970·Molecular & General Genetics : MGG·M WimanI Sasaki
Dec 19, 2018·BMC Systems Biology·Gabriela I GuzmánAdam M Feist
Jul 4, 2019·Amino Acids·Daniel Charlier, Indra Bervoets
Nov 5, 2014·Biological Reviews of the Cambridge Philosophical Society·Yosef FichmanLaszlo N Csonka
Dec 1, 2004·EcoSal Plus·Daniel Charlier, Nicolas Glansdorff
Dec 1, 1972·Bacteriological Reviews·K E Sanderson
Mar 1, 1975·Journal of Bacteriology·C M BergM Conlon
Jun 1, 1970·Bacteriological Reviews·K E Sanderson
Dec 1, 1974·Journal of Bacteriology·S T Chung, G R Greenberg
Sep 1, 1986·Microbiological Reviews·R CuninV Stalon
Apr 1, 2007·EcoSal Plus·Laszlo N Csonka, Thomas Leisinger
Nov 1, 1976·Journal of Bacteriology·R A Kelln, G A O'Donovan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.