Suppression of protein expression of three avian reovirus S-class genome segments by RNA interference

Veterinary Microbiology
Wen T JiHung J Liu

Abstract

RNA interference was used to suppress protein expression of three S-class genome segments of avian reovirus (ARV). Viral progeny titer was successfully down-regulated by RNA interference. Suppression of S1 genome segment, which has three open reading frames, not only decreased the expression level of the structural protein sigmaC but also reduced cell fusion and the level of Ser(15)-phosphorylated p53 protein caused by the nonstructural proteins p10 and p17, respectively. Suppression of S2 or S4 genome segment by RNA interference could also reduce the expression level of sigmaA or sigmaNS. Interestingly, suppression of sigmaNS resulted in down regulation of the expression of other viral products. In terms of variability of different genes among viral strains and of the impact after their suppression, it seems that the viral products involved in construction of viroplasm or core particles, like sigmaNS, are considerable choices to efficiently inhibit ARV multiplication by RNA interference. Using a GFP reporter system, it was discovered that ARV could not inhibit activated RNA interference, suggesting that RNA interference may be used in the suppression of ARV infection.

References

May 23, 2002·Science·Hongwei LiShou Wei Ding
Nov 26, 2002·EMBO Reports·Miguel Angel DéctorCarlos F Arias
Mar 26, 2003·The Journal of General Virology·Zsuzsanna LichnerJózsef Burgyán
Feb 6, 2004·Nucleic Acids Research·Koichi YoshinariKazunari Taira
Mar 23, 2004·Virology·Wen L ShihHung J Liu
Feb 3, 2005·Nucleic Acids Research·Ellen M WesterhoutBen Berkhout
Mar 24, 2005·The Journal of General Virology·Fernando Tourís-OteroJavier Benavente
Jul 15, 2005·Journal of Virology·M Gunnar AnderssonGöran Akusjärvi
Sep 7, 2005·Biochemical and Biophysical Research Communications·Hung-Jen LiuWen-Ling Shih
Oct 5, 2006·Virus Research·Javier Benavente, Jose Martínez-Costas
Mar 24, 2007·Biochemical and Biophysical Research Communications·Julius L C ChuluHung J Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis