Suppression of reactive oxygen species generation in heart mitochondria from anoxic turtles: the role of complex I S-nitrosation.

The Journal of Experimental Biology
Amanda BundgaardAngela Fago

Abstract

Freshwater turtles (Trachemys scripta) are among the very few vertebrates capable of tolerating severe hypoxia and re-oxygenation without suffering from damage to the heart. As myocardial ischemia and reperfusion causes a burst of mitochondrial reactive oxygen species (ROS) in mammals, the question arises as to whether, and if so how, this ROS burst is prevented in the turtle heart. We find that heart mitochondria isolated from turtles acclimated to anoxia produce less ROS than mitochondria from normoxic turtles when consuming succinate. As succinate accumulates in the hypoxic heart and is oxidized when oxygen returns, this suggests an adaptation to lessen ROS production. Specific S-nitrosation of complex I can lower ROS in mammals and here we show that turtle complex I activity and ROS production can also be strongly depressed in vitro by S-nitrosation. We detect in vivo endogenous S-nitrosated complex I in turtle heart mitochondria, but these levels are unaffected upon anoxia acclimation. Thus, while heart mitochondria from anoxia-acclimated turtles generate less ROS and have a lower aerobic capacity than those from normoxic turtles, this is not due to decreases in complex I activity or expression levels. Interestingly, in-ge...Continue Reading

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Citations

Feb 23, 2019·The Journal of Experimental Biology·William JoyceTobias Wang
May 13, 2020·The Journal of Experimental Biology·Amanda BundgaardAngela Fago
Jul 12, 2020·The Journal of Experimental Biology·Georgina K Cox, Todd E Gillis
May 22, 2019·The Journal of Experimental Biology·Birgitte JensenAngela Fago
Jan 1, 2021·Free Radical Biology & Medicine·Chidozie N OkoyeCollins Kamunde
Dec 5, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Amanda BundgaardAngela Fago
Aug 11, 2020·Gene·Rasha Al-Attar, Kenneth B Storey
Mar 25, 2021·The Journal of Experimental Biology·Jakob MichaelsenAmanda Bundgaard
May 29, 2021·The Journal of Experimental Biology·Varshinie PillaiEbrahim Lari
May 26, 2021·The Science of the Total Environment·Abraão Tiago Batista Guimarães, Guilherme Malafaia
Jun 20, 2021·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Michael O IseiCollins Kamunde
Dec 7, 2021·The Journals of Gerontology. Series A, Biological Sciences and Medical Sciences·Enrique RodríguezPierre U Blier

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