Suppression of sensitivity to drugs and antibiotics by high external cation concentrations in fission yeast

PloS One
John P AlaoPer Sunnerhagen

Abstract

Potassium ion homeostasis plays an important role in regulating membrane potential and therefore resistance to cations, antibiotics and chemotherapeutic agents in Schizosaccharomyces pombe and other yeasts. However, the precise relationship between drug resistance in S. pombe and external potassium concentrations (particularly in its natural habitats) remains unclear. S. pombe can tolerate a wide range of external potassium concentrations which in turn affect plasma membrane polarization. We thus hypothesized that high external potassium concentrations suppress the sensitivity of this yeast to various drugs. We have investigated the effect of external KCl concentrations on the sensitivity of S. pombe cells to a wide range of antibiotics, antimicrobial agents and chemotherapeutic drugs. We employed survival assays, immunoblotting and microscopy for these studies. We demonstrate that KCl, and to a lesser extent NaCl and RbCl can suppress the sensitivity of S. pombe to a wide range of antibiotics. Ammonium chloride and potassium hydrogen sulphate also suppressed drug sensitivity. This effect appears to depend in part on changes to membrane polarization and membrane transport proteins. Interestingly, we have found little relationsh...Continue Reading

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Citations

Jan 23, 2016·Scientific Reports·Thi Thuy Trang NguyenEe Sin Chen
Aug 6, 2021·Frontiers in Microbiology·Sandra MoroM-Henar Valdivieso

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Methods Mentioned

BETA
fluorescence microscopy
environmental stresses
florescence microscopy

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