Suppression of sialyl Lewis X expression and E-selectin-mediated cell adhesion in cultured human lymphoid cells by transfection of antisense cDNA of an alpha1-->3 fucosyltransferase (Fuc-T VII).

The Journal of Biological Chemistry
N HiraiwaR Kannagi

Abstract

The antisense cDNA approach was used to identify the endogenous fucosyltransferase species responsible for synthesis of the sialyl Lewis X (NeuAcalpha2-->3 Galbeta1-->4[Fucalpha1-->3]GlcNAcbeta1-->R) determinant in human lymphoid cells. The cultured human adult T-cell leukemia cell line, ED40515-N, expressed the message of alpha1-->3 fucosyltransferase (Fuc-T) IV and VII, with a low level of the Fuc-T III and VI message, and manifested the sialyl Lewis X as well as Lewis X (Galbeta1-->4 [Fucalpha1-->3]GlcNAcbeta1-->R) determinant at the cell surface. Transfection of this cell line with the pRc/CMV vector containing an antisense human Fuc-T VII construct (pRc/CMV/5'FT7AS) resulted in a significant decrease of endogenous Fuc-T VII message and a marked reduction in the cell surface expression of sialyl Lewis X determinant as well as a reduction in the enzymatic activity of alpha1-->3 fucosyltransferase against sialylated type 2 chain substrate. This was accompanied by diminution of cell adhesive activity toward E-selectin on interleukin-1beta-treated endothelial cells. These results indicated that the synthesis of the sialyl Lewis X determinants that were functionally active as E-selectin ligands was mainly mediated by Fuc-T VII i...Continue Reading

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Citations

Aug 6, 1999·Biochimica Et Biophysica Acta·T PetrettiW Kemmner
Dec 2, 1999·Biochimica Et Biophysica Acta·E StaudacherL März
Apr 16, 1998·International Journal of Urology : Official Journal of the Japanese Urological Association·M SatohS Orikasa
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May 11, 2004·Cancer Science·Reiji KannagiNaoko Kimura
Apr 15, 2004·Archives of Biochemistry and Biophysics·Xiaoda NiuDaniel Lundell
Jun 19, 2001·The Journal of Biological Chemistry·P BengtsonP Påhlsson
Jun 6, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sima AllahverdianDelbert R Dorscheid
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Sep 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Per BengtsonPeter Påhlsson
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Apr 30, 2002·Experimental Cell Research·Shaoqiang LinPeter M Schlag
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