PMID: 36149Apr 26, 1979

Suppression of the accumulation of steroid-inducible glutamine synthetase mRNA on embryonic chick retinal polysomes by interferon preparation

Biochimica Et Biophysica Acta
N Shirasawa, T Matsuno

Abstract

The suppression mechanism of glutamine synthetase [EC 6.3.1.2] induction mediated by chick interferon preparation was investigated in embryonic chick neural retina cell cultures. A translational assay was used to measure the level of mRNA coding for the enzyme. RNA extracted from the retinal polysomes was chromatographed on oligo(dT)-cellulose and translated in a cell-free protein synthesizing system derived from wheat germ. The newly synthesized enzyme was isolated by immunoprecipitation with anti-enzyme gamma-globulin and identified using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Neither polysomal profiles nor total protein synthesis directed by the RNA preparations was affected by interferon treatment. Comparing the amount of glutamine synthetase synthesized in vitro by equal amounts of mRNA fraction from retinas treated with or without interferon indicated that the suppression was associated with reduced level of the enzyme mRNA on polysomes.

References

Jan 1, 1987·Cancer Metastasis Reviews·M Shearer, J Taylor-Papadimitriou

Citations

May 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·E De MaeyerM Vandeputte
May 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G B RossiF Belardelli
Nov 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·D L Kirk
Jun 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·H Aviv, P Leder
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·B E Roberts, B M Paterson

Related Concepts

Embryo
Polyribosomes
Precipitin Tests
Set of Polysomal Ribosomes
Organ Culture Techniques
GLUL
SDS-PAGE
Cell Culture Techniques
Glutamate-Ammonia Ligase
Human leukocyte interferon

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