Suppression of the cell proliferation in stomach cancer cells by the ZNRD1 gene

Biochemical and Biophysical Research Communications
L HongDai-ming Fan

Abstract

Zinc ribbon domain-containing 1 (ZNRD1), a transcription-associated gene, was recently found to be downregulated in human gastric cancer tissues as compared to the matched adjacent nonneoplastic tissues. In this study, we constructed the siRNA eukaryotic expression vectors of ZNRD1 and transfected them into normal gastric epithelial cells (GES-1). We also introduced the ZNRD1 gene into gastric cancer cells that do (SGC7901) and do not (AGS) express ZNRD1 endogenously. GES-1 cells stably transfected with the ZNRD1-RNAi were found to exhibit significantly quicker proliferation than empty vector transfectants. AGS cells stably transfected with the ZNRD1 cDNA exhibited significantly decreased growth rate as compared to control vector transfectants, whereas SGC7901 cells did not. Furthermore, ZNRD1 suppresses growth of AGS cells in soft agar and tumor formation in athymic nude mice. This study clearly demonstrates that ZNRD1 may play an important role in the control of human gastric cancer development by regulating cell proliferation. These results provide new insights into the function of ZNRD1 and further validate ZNRD1 as a potential therapeutic target in gastric cancer.

References

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Citations

Feb 18, 2014·International Journal of Biological Macromolecules·Ping ZhangHui Ji
Mar 3, 2005·Journal of Gastroenterology and Hepatology·Daiming FanKaichun Wu
Jul 3, 2008·Digestive Diseases and Sciences·Yunping ZhaoDaiming Fan
Dec 23, 2008·International Journal of Pediatric Otorhinolaryngology·Yu HanJianhua Qiu
Nov 18, 2010·Cell Biology International·Liu HongDaiming Fan
Jan 16, 2007·Neoplasia : an International Journal for Oncology Research·Jiugang SongDaiming Fan
Apr 13, 2006·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Liu HongDaiming Fan

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