Suppression of tubulin detyrosination by parthenolide recruits the plant-specific kinesin KCH to cortical microtubules

Journal of Experimental Botany
Natalie SchneiderPeter Nick

Abstract

Detyrosination of α-tubulin seems to be conserved in all eukaryotes. However, its biological function in plants has remained obscure. A conserved C-terminal tyrosine is removed by a still unidentified tubulin-tyrosine carboxypeptidase (TTC) and can be religated by a tubulin-tyrosine ligase (TTL). To obtain insight into the still elusive biological function of this detyrosination-tyrosination cycle, the effects of the TTC inhibitor parthenolide were analysed in BY-2 tobacco cells. Parthenolide caused a depletion of detyrosinated α-tubulin, whereas the level of tyrosinated tubulin was elevated. This biochemical effect was accompanied by growth inhibition in cycling BY-2 cells and alteration of microtubule-dependent events that define division and expansion geometry such as cell plate alignment or axial expansion. Furthermore, parthenolide triggered an apoplastic alkalinization indicative of activation of defence-related calcium influx channels. At the same time, parthenolide promoted the association of the plant-specific kinesin KCH with cortical microtubules. These observations are integrated into a working model, where detyrosination acts as signal to modulate the binding of kinesin motors involved in structural and sensory fun...Continue Reading

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Citations

Nov 12, 2015·Current Opinion in Plant Biology·Yuh-Ru Julie LeeBo Liu
Feb 24, 2016·Journal of Integrative Plant Biology·Linda BrochhausenPeter Nick
Nov 26, 2019·Natural Product Reports·Robert R A FreundHans-Dieter Arndt
Oct 1, 2019·Plant Signaling & Behavior·John Gardiner
Jan 23, 2021·Current Biology : CB·Takashi HottaRyoma Ohi
Dec 19, 2020·Journal of Integrative Plant Biology·Kunxi ZhangPeter Nick

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