Suppression of type I interferon production by porcine epidemic diarrhea virus and degradation of CREB-binding protein by nsp1

Virology
Qingzhan ZhangDongwan Yoo

Abstract

Type I interferons (IFN-α/β) are the major components of the innate immune response of hosts, and in turn many viruses have evolved to modulate the host response during infection. We found that the IFN-β production was significantly suppressed during PEDV infection in cells. To identify viral IFN antagonists and to study their suppressive function, viral coding sequences for the entire structural and nonstructural proteins were cloned and expressed. Of 16 PEDV nonstructural proteins (nsps), nsp1, nsp3, nsp7, nsp14, nsp15 and nsp16 were found to inhibit the IFN-β and IRF3 promoter activities. The sole accessory protein ORF3, structure protein envelope (E), membrane (M), and nucleocapsid (N) protein were also shown to inhibit such activities. PEDV nsp1 did not interfere the IRF3 phosphorylation and nuclear translocation but interrupted the enhanceosome assembly of IRF3 and CREB-binding protein (CBP) by degrading CBP. A further study showed that the CBP degradation by nsp1 was proteasome-dependent. Our data demonstrate that PEDV modulates the host innate immune responses by degrading CBP and suppressing ISGs expression.

References

Mar 19, 1977·The Veterinary Record·E N Wood
Nov 1, 1988·Journal of Clinical Microbiology·M Hofmann, R Wyler
Apr 1, 1981·Infection and Immunity·C La Bonnardiere, H Laude
Jul 1, 1995·Journal of Comparative Pathology·M SueyoshiY Suzuki
Jan 1, 1993·Advances in Experimental Medicine and Biology·M DuarteH Laude
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·V J LaMorteR M Evans
Aug 26, 1998·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·E AlbinaB Charley
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·V DoucasR M Evans
Mar 10, 2001·The Journal of Cell Biology·F M BoisvertD P Bazett-Jones
Nov 13, 2001·Oncogene·K JensenP S Freemont
Jan 5, 2002·Journal of Virology·Fumihiro Taguchi, Shutoku Matsuyama
Jan 19, 2002·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·J PeiE W Collisson
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 15, 2003·Nature Immunology·Katherine A FitzgeraldTom Maniatis
Dec 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jianyin LongFang Liu
Jun 4, 2004·Expert Opinion on Biological Therapy·Jindrich CinatlHans Wilhelm Doerr
Oct 28, 2004·Veterinary Immunology and Immunopathology·Sang-Myeong LeeSteven B Kleiboeker
Dec 8, 2004·Virology·Stephanie JenningsGeorg Kochs
Dec 21, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aitor G GranjaYolanda Revilla
Aug 26, 2006·Nature Reviews. Immunology·Kenya Honda, Tadatsugu Taniguchi
Jun 19, 2007·Cell·Daniel PanneStephen C Harrison
Nov 18, 2008·Epigenetics : Official Journal of the DNA Methylation Society·Jonathan R St-GermainQiao Li
Jul 25, 2009·Emerging Infectious Diseases·Suphasawatt PuranavejaRoongroje Thanawongnuwech
Jul 9, 2010·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yongjin WangTianhou Wang

❮ Previous
Next ❯

Citations

May 24, 2016·Virus Research·Samaporn TeeravechyanAnan Jongkaewwattana
Jul 12, 2016·Virus Research·Sonia ZuñigaLuis Enjuanes
Jul 8, 2016·Viruses·To Sing FungDing Xiang Liu
Jul 30, 2020·PLoS Pathogens·Margarida Sa RiberoSébastien Nisole
Mar 12, 2019·Frontiers in Veterinary Science·Surapong KoonpaewAnan Jongkaewwattana
Nov 29, 2016·Frontiers in Immunology·Katharina S Schulz, Karen L Mossman
Jan 24, 2019·Frontiers in Immunology·Hao-Sen Chiang, Helene Minyi Liu
May 15, 2020·Pathogens·Shasha LiHaixue Zheng
May 29, 2020·Journal of Virology·Xinyu ZhuShaobo Xiao
Nov 6, 2020·The Journal of General Virology·Zhou ShenGuiqing Peng
Nov 23, 2020·International Immunopharmacology·Mohadeseh Haji AbdolvahabLeila Farahmand
Feb 3, 2021·International Journal of Molecular Sciences·Nicholas A Wong, Milton H Saier

❮ Previous
Next ❯

Datasets Mentioned

BETA
KF272920

Methods Mentioned

BETA
confocal microscopy
nuclear translocation
co-immunoprecipitation
co-IP
transfection
IECs
ubiquitination
PCR
fluorescence microscopy
protein assay

Software Mentioned

LSM image browser

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.