PMID: 9191021May 1, 1997Paper

Suppressive effects of destruxin B on hepatitis B virus surface antigen gene expression in human hepatoma cells

Antiviral Research
H C ChenS F Yeh

Abstract

Destruxin B, a cyclodepsipeptide was originally identified as a plant pathogen from the fungus, Alternaria brassicae. We examined the antiviral activity of destruxin B and found that it suppresses the expression of the hepatitis B viral surface antigen (HBsAg) gene in human hepatoma Hep3B cells which carry an integrated viral gene in its chromosome. In contrast, destruxin B shows no cytotoxic effect on the viability of the cells. Furthermore, it can be shown that destruxin B can reversibly suppress HBsAg production by Hep3B cells in a concentration-dependent manner with EC50 of 0.5 microM. Northern blot analysis indicates that the suppression of HBsAg gene expression by destruxin B is mainly at the mRNA level. Destruxin B not only suppresses the endogenously expressed HBsAg in the Hep3B cells but also suppresses the HBsAg produced either from the stable transfected HBV DNA in another human hepatoma HuH-7 cell line which carry no endogenous HBV genome. These results suggest that destruxin B may have future potential for development as a specific anti-HBV drug.

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Citations

Feb 28, 2002·Phytochemistry·M Soledade C PedrasDale E Ward
Jan 11, 2012·Proceedings of the National Academy of Sciences of the United States of America·Bing WangChengshu Wang
Dec 5, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Yu-Chuen HuangYu-Yawn Chen
Aug 21, 2013·Insect Science·Xiu-Run ChenShun-Xiang Ren
Nov 15, 2011·Biotechnology Advances·Bing-Lan Liu, Yew-Min Tzeng
Jun 12, 2013·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Pin-Zhir ChaoYung-Feng Lin
Jan 18, 2014·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Thanh-Tuan HuynhChi-Tai Yeh
Jun 29, 2014·BMC Complementary and Alternative Medicine·Rosa Huang LiuYew-Min Tzeng
Mar 20, 2010·Toxicon : Official Journal of the International Society on Toxinology·Augusto Schrank, Marilene Henning Vainstein
Apr 11, 2014·Archives of Insect Biochemistry and Physiology·Jiqiao FanMingqiang Ye
Dec 29, 2006·Chemistry & Biodiversity·María J VázquezJuan A Hueso-Rodríguez
Apr 29, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shuo TianHong Chen

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