Suppressive effects of sirtinol on human cytomegalovirus (hCMV) infection and hCMV-induced activation of molecular mechanisms of senescence and production of reactive oxygen species

Mechanisms of Ageing and Development
Genxiang MaoSean X Leng

Abstract

Substantial evidence suggests that chronic human cytomegalovirus (hCMV) infection contributes significantly to T-cell immunosenescence and adverse health outcomes in older adults. As such, it is important to search for compounds with anti-hCMV properties. Studies have shown that resveratrol, a sirtuin activator, suppresses hCMV infection. Here we report suppressive effects of sirtinol, a sirtuin antagonist, on hCMV infection and its cellular and molecular consequences. Human diploid fibroblast WI-38 cells were infected by hCMV Towne strain in the absence or presence of sirtinol. hCMV replication was measured using qPCR. Senescent phenotype was determined by senescence-associated β galactosidase (SA-β-Gal) activity. Expression of hCMV immediate early (IE) and early (E) proteins and senescence-associated proteins (pRb and Rb, p16(INK4), and p53) and production of reactive oxygen species (ROS) were assessed using standard laboratory assays. The results demonstrated that sirtinol suppressed hCMV infection as well as hCMV-induced activation of molecular mechanisms of senescence and ROS production. While underlying molecular mechanisms remain to be elucidated, these findings indicate sirtinol as a novel and potent anti-hCMV agent wit...Continue Reading

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Citations

Aug 16, 2016·Mechanisms of Ageing and Development·Graham Pawelec
Oct 23, 2016·Fish & Shellfish Immunology·Junhui ShaoJunfa Yuan
May 4, 2017·Antioxidants & Redox Signaling·Luis Ángel Maciel-BarónMina Königsberg
Nov 2, 2017·Cell & Bioscience·Qing YeQuanxing Wang
Jul 11, 2019·MBio·Brent A HackettSara Cherry
Jun 12, 2020·The Journal of Microbiology·San-Ying WangGen-Xiang Mao
Jul 7, 2017·GeroScience·Sean X LengFelicia D Goodrum
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Apr 6, 2021·Current Clinical Microbiology Reports·Chandler H Monk, Kevin J Zwezdaryk
Apr 14, 2021·Laboratory Investigation; a Journal of Technical Methods and Pathology·Hyunju KangJi-Young Lee

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