PMID: 11329618May 1, 2001Paper

Suppressive role of endogenous regucalcin in the regulation of protein phosphatase activity in rat renal cortex cytosol

Journal of Cellular Biochemistry
Yoshiko Morooka, Masayoshi Yamaguchi

Abstract

The role of endogenous regucalcin, which is a regulatory protein of calcium signaling, in the regulation of protein phosphatase activity in the cytosol of rat renal cortex was investigated. Protein phosphatase activity toward phosphotyrosine, phosphoserine, and phosphothreonine was found in the cytosol of kidney cortex. The addition of regucalcin (50-250 nM) in the enzyme reaction mixture caused a significant decrease in protein phosphatase activity toward three phosphoamino acids. The effect of calcium (25 microM) and calmodulin (2.5 microg/ml) in increasing protein phosphatase activity toward three phosphoamino acids was significantly decreased by the addition of regucalcin (100 nM). Protein phosphatase activity toward three phosphoamino acids was significantly increased in the presence of anti-regucalcin monoclonal antibody (10-50 ng/ml) in the enzyme reaction mixture. The effect of antibody (25 ng/ml) in increasing the enzyme activity was significantly inhibited by cyclosporin A (10(-5) M) or vanadate (10(-5) M). Regucalcin in the kidney cortex cytosol was clearly decreased by the administration of saline (0.9% NaCl) for seven days in rats. Protein phosphatase activity toward three phosphoamino acids was significantly decre...Continue Reading

References

Jun 1, 1978·Chemical & Pharmaceutical Bulletin·M Yamaguchi, T Yamamoto
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·D A FrumanS J Burakoff
Mar 1, 1991·Trends in Biochemical Sciences·C W Heizmann, W Hunziker
Oct 27, 1995·The Journal of Biological Chemistry·Y WangC Y Huang
May 12, 1993·Molecular and Cellular Biochemistry·M Yamaguchi, M Isogai
Mar 1, 1996·Metabolism: Clinical and Experimental·N Kraus-Friedmann, L Feng
May 16, 2000·Life Sciences·M Yamaguchi
Sep 28, 2000·Biochemical and Biophysical Research Communications·M Yamaguchi

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Citations

Sep 2, 2004·Biological & Pharmaceutical Bulletin·Yuko Fukaya, Masayoshi Yamaguchi
Mar 23, 2013·Cellular and Molecular Life Sciences : CMLS·Ricardo MarquesSílvia Socorro
Sep 24, 2015·International Journal of Molecular Medicine·Masayoshi Yamaguchi
Aug 14, 2001·Journal of Cellular Biochemistry·Y Morooka, M Yamaguchi
Mar 25, 2011·Molecular and Cellular Biochemistry·Masayoshi Yamaguchi

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