Suppressor capacity of copper nanoparticles biosynthesized using Crocus sativus L. leaf aqueous extract on methadone-induced cell death in adrenal phaeochromocytoma (PC12) cell line.

Scientific Reports
Peng ZhangFatih Şen

Abstract

In this research, we prepared and formulated a neuroprotective supplement (copper nanoparticles in aqueous medium utilizing Crocus sativus L. Leaf aqueous extract) for determining its potential against methadone-induced cell death in PC12. The results of chemical characterization tests i.e., FE-SEM, FT-IR, XRD, EDX, TEM, and UV-Vis spectroscopy revealed that the study showed that copper nanoparticles were synthesized in the perfect way possible. In the TEM and FE-SEM images, the copper nanoparticles were in the mean size of 27.5 nm with the spherical shape. In the biological part of the present research, the Rat inflammatory cytokine assay kit was used to measure the concentrations of inflammatory cytokines. Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) test was used to show DNA fragmentation and apoptosis. Caspase-3 activity was assessed by the caspase activity colorimetric assay kit and mitochondrial membrane potential was studied by Rhodamine123 fluorescence dye. Also, the cell viability of PC12 was measured by trypan blue assay. Copper nanoparticles-treated cell cutlers significantly (p ≤ 0.01) decreased the inflammatory cytokines concentrations, caspase-3 activity, and DNA fragmentation and they rais...Continue Reading

References

Jun 1, 1992·Journal of Pharmaceutical Sciences·F ReigM A Alsina
Mar 31, 2000·Experimental Cell Research·S H Kaufmann, W C Earnshaw
Oct 26, 2000·Nature·M O Hengartner
Jun 14, 2002·Drug and Alcohol Dependence·Miriam Z Mintzer, Maxine L Stitzer
Mar 30, 2004·Annals of Neurology·Michael H Barnett, John W Prineas
Oct 29, 2005·Fitoterapia·Hossein Hosseinzadeh, Fatemeh Talebzadeh
Jan 12, 2007·Biochimica Et Biophysica Acta·Takashi OchiaiShinji Soeda
Jul 2, 2009·Mayo Clinic Proceedings·Howard S Smith
Jul 3, 2009·International Journal of Clinical Practice·W Leppert
Sep 1, 2009·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Maria Luisa SotgiuGabriele E M Biella
Oct 8, 2009·Journal of Child Neurology·Anna ZaninAnna Maria Laverda
Aug 10, 2010·Biochimica Et Biophysica Acta·Sergio Perez-AlvarezJoaquin Jordán
Oct 28, 2010·Journal of Neuroimaging : Official Journal of the American Society of Neuroimaging·Alfonso CeraseCarlo Venturi
Jan 18, 2011·Behavioural Brain Research·Magdalini A PapandreouMarigoula Margarity
Mar 25, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nidia QuillinanJohn T Williams
Oct 4, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·André M DeslauriersChristopher Power
Jul 1, 2010·Pharmacognosy Reviews·R SrivastavaS A Saraf
Feb 22, 2012·Nanomedicine·William J TricklerSyed F Ali
Feb 20, 2014·Experimental and Clinical Psychopharmacology·Olga RassMiriam Z Mintzer
Oct 5, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Renu SankarVilwanathan Ravikumar
Feb 11, 2015·Psychiatry Research·Yuan-Yu ChanWan-Yu Lo
Nov 4, 2015·Current Protocols in Immunology·Warren Strober
Mar 16, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dilaveez RehanaA Kalilur Rahiman

❮ Previous
Next ❯

Methods Mentioned

BETA
surface plasmon resonance
X-ray

Software Mentioned

ImageJ

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis