PMID: 9555883Apr 29, 1998Paper

Suppressor mutation analysis of the sensory rhodopsin I-transducer complex: insights into the color-sensing mechanism

Journal of Bacteriology
K H Jung, J L Spudich

Abstract

The molecular complex containing the phototaxis receptor sensory rhodopsin I (SRI) and transducer protein HtrI (halobacterial transducer for SRI) mediates color-sensitive phototaxis responses in the archaeon Halobacterium salinarum. One-photon excitation of the complex by orange light elicits attractant responses, while two-photon excitation (orange followed by near-UV light) elicits repellent responses in swimming cells. Several mutations in SRI and HtrI cause an unusual mutant phenotype, called orange-light-inverted signaling, in which the cell produces a repellent response to normally attractant light. We applied a selection procedure for intragenic and extragenic suppressors of orange-light-inverted mutants and identified 15 distinct second-site mutations that restore the attractant response. Two of the 3 suppressor mutations in SRI are positioned at the cytoplasmic ends of helices F and G, and 12 suppressor mutations in HtrI cluster at the cytoplasmic end of the second HtrI transmembrane helix (TM2). Nearly all suppressors invert the normally repellent response to two-photon stimulation to an attractant response when they are expressed with their suppressible mutant alleles or in an otherwise wild-type strain. The results ...Continue Reading

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Citations

Mar 4, 2006·Journal of Bacteriology·Kylie J WattsBarry L Taylor
Nov 26, 2010·Journal of Bacteriology·Asharie J CampbellBarry L Taylor
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Feb 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·X N ZhangJ L Spudich
Oct 14, 2000·Annual Review of Cell and Developmental Biology·J L SpudichE N Spudich
Jun 25, 2003·The Journal of Biological Chemistry·Vladislav BergoKenneth J Rothschild
Jan 5, 2002·Antioxidants & Redox Signaling·B L TaylorM S Johnson
Oct 10, 2001·Journal of Bacteriology·K H JungJ L Spudich
Oct 19, 2004·Journal of Bacteriology·Kylie J WattsBarry L Taylor
Apr 2, 2011·The Journal of Biological Chemistry·Jun SasakiJohn L Spudich
Jun 21, 2008·The Journal of Biological Chemistry·Tomomi Kitajima-IharaYuki Sudo
Nov 5, 1999·Annual Review of Microbiology·B L TaylorM S Johnson
Oct 15, 2008·Proceedings of the National Academy of Sciences of the United States of America·Oleg A SineshchekovJohn L Spudich
Mar 17, 2017·Annual Review of Biochemistry·Elena G GovorunovaJohn L Spudich
Dec 28, 1999·Methods : a Companion to Methods in Enzymology·M D Manson
Sep 14, 2000·Biochimica Et Biophysica Acta·J Sasaki, J L Spudich
Jul 2, 2010·Biochemistry·Oleg A SineshchekovJohn L Spudich
Oct 8, 1999·Biophysical Journal·J Sasaki, J L Spudich

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