Nov 1, 1995

Suppressor of hairless directly activates transcription of enhancer of split complex genes in response to Notch receptor activity

Genes & Development
A M Bailey, J W Posakony

Abstract

We have investigated the functional relationships among three loci that are required for multiple alternative cell fate decisions during adult peripheral neurogenesis in Drosophila: Notch (N), which encodes a transmembrane receptor protein, Suppressor of Hairless [Su(H)], which encodes a DNA-binding transcription factor, and the Enhancer of split gene complex [E(spl)-C], which includes seven transcription units that encode basic helix-loop-helix (bHLH) repressor proteins. We describe several lines of evidence establishing that Su(H) directly activates transcription of E(spl)-C genes in response to N receptor activity. Expression of an activated form of the N receptor leads to elevated and ectopic E(spl)-C transcript accumulation and promoter activity in imaginal discs. We show that the proximal upstream regions of three E(spl)-C genes contain multiple specific binding sites for Su(H). The integrity of these sites, as well as Su(H) gene activity, are required not only for normal levels of expression of E(spl)-C genes in imaginal disc proneural clusters, but also for their transcriptional response to hyperactivity of the N receptor. Our results establish Su(H) as a direct regulatory link between N receptor activity and the expres...Continue Reading

  • References
  • Citations308

References

  • We're still populating references for this paper, please check back later.
  • References
  • Citations308

Mentioned in this Paper

Receptors, Notch
Conserved Sequence
Suppressor of Hairless protein, Drosophila
Molecular Helix
Cell Fate
Notch protein, Drosophila
Notch
Drosophila melanogaster Proteins
Transcription, Genetic
Peripheral Nervous System

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.