Abstract
In normal adult human skin, expression of epidermal integrins is confined to keratinocytes in the basal layer. However, suprabasal expression of alpha 2, alpha 3 and beta 1 integrin subunits is noted in hyperproliferative epidermis in wound repair and psoriasis. In this study, we examined the effect of topical all-trans-retinoic acid (RA), known to induce epidermal hyperplasia, on expression of integrins in human epidermis. Immunostaining of vehicle-treated skin revealed expression of alpha 2, alpha 3 and beta 1, as well as alpha 6 and beta 4 integrin subunits entirely on basal keratinocytes. Topical application of RA (0.1%) for 2 weeks resulted in marked suprabasal expression of alpha 2, alpha 3 and beta 1 integrin subunits, whereas alpha 6 and beta 4 staining remained on basal keratinocytes. Staining for putative ligands of alpha 2 beta 1 and alpha 3 beta 1 integrins, i.e. type IV collagen, laminin-5 and fibronectin, was not detected in the epidermal layer in RA- or vehicle-treated skin. Treatment of HaCaT keratinocytes in culture with RA (1 mumol/L) enhanced alpha 2 and beta 1 mRNA abundance. Furthermore, RA slightly up-regulated the expression of alpha 2, alpha 3 and beta 1 integrin subunits on primary epidermal keratinocyt...Continue Reading
References
Nov 27, 1979·Biochemistry·J M ChirgwinW J Rutter
Mar 1, 1992·The Journal of Investigative Dermatology·D S RosenthalJ J Voorhees
Mar 1, 1992·The Journal of Investigative Dermatology·H J KimG F Bryce
Jun 1, 1992·The Journal of Clinical Investigation·M D HertleF M Watt
Sep 1, 1991·The British Journal of Dermatology·I KellnerW Sterry
May 1, 1991·The Journal of Investigative Dermatology·G J FisherB J Nickoloff
Mar 1, 1990·The Journal of Cell Biology·H LarjavaK M Yamada
Jun 1, 1991·The British Journal of Dermatology·E RalfkiaerG L Vejlsgaard
Apr 1, 1991·Journal of Biochemistry·T TsujiT Osawa
Jan 1, 1992·The Journal of Investigative Dermatology·G J FisherL Ellingsworth
Jan 1, 1991·The American Journal of the Medical Sciences·H Larjava
May 1, 1987·Experimental Cell Research·P VerrandoJ P Ortonne
Mar 1, 1988·The Journal of Cell Biology·P BoukampN E Fusenig
Mar 1, 1989·The Journal of Investigative Dermatology·R TammiM Tammi
Oct 1, 1989·The Journal of Investigative Dermatology·J VaraniJ J Voorhees
Aug 1, 1989·The Journal of Cell Biology·S K AkiyamaK M Yamada
Jul 1, 1989·The Journal of Cell Biology·Y Takada, M E Hemler
Dec 1, 1989·The Journal of Clinical Investigation·J PeltonenJ Uitto
Oct 1, 1989·Analytical Biochemistry·W KuengU Eppenberger
Sep 1, 1987·The Journal of Cell Biology·W S ArgravesE Ruoslahti
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·W J RettigL J Old
Apr 1, 1995·The Journal of Investigative Dermatology·E Schwartz, L H Kligman
May 1, 1995·The Journal of Investigative Dermatology·S ChenR Sterzycki
Jan 1, 1995·The British Journal of Dermatology·C PoppT J Stoudemayer
Jun 2, 1995·The Journal of Biological Chemistry·T RiikonenJ Heino
Feb 1, 1995·The Journal of Investigative Dermatology·M D HertleF M Watt
Nov 1, 1994·The Journal of Clinical Investigation·J VaraniJ J Voorhees
Aug 1, 1994·The Journal of Investigative Dermatology·J GailitR A Clark
Dec 1, 1993·The Journal of Clinical Investigation·U K Saarialho-KereW C Parks
Sep 1, 1993·The Journal of Clinical Investigation·H LarjavaJ Heino
Dec 15, 1995·Cell·J M CarrollF M Watt
Mar 15, 1996·International Journal of Cancer. Journal International Du Cancer·J Heino
Citations
Mar 5, 2005·Eye·A M Abu El-AsrarK Geboes
Aug 22, 2006·Basic & Clinical Pharmacology & Toxicology·Thomas K Petersen
Nov 3, 2011·PloS One·Thuy TranMary Zutter
Apr 25, 2013·PloS One·Elisabeth Gustafson-Wagner, Christopher S Stipp
May 21, 2014·ELife·Sara CipolatFiona M Watt
May 14, 2011·Postgraduate Medicine·Peter HsuRobert T Brodell
Nov 16, 2018·Biology Open·Minna PiipponenLiisa Nissinen
May 16, 2002·European Journal of Oral Sciences·Christoph BeckerHans Georg Gräber
Nov 12, 2019·Bioscience Reports·Yong-Zhi Ji, Shi-Rui Liu
Apr 30, 2017·International Journal of Immunopathology and Pharmacology·Emale El DarziGeorges M Bahr