Supramolecular Host-Guest Chemistry-Based Folate/Riboflavin Functionalization and Cancer Cell Labeling of Nanoparticles

ACS Omega
Suman PalNikhil R Jana

Abstract

Nanoparticle-based cellular probes are commonly designed via covalent conjugation with affinity biomolecules. Those nanobioconjugates selectively interact with cell surface receptors and induce endocytosis followed by intracellular trafficking. However, this approach requires functional modification of biomolecules that may alter their biochemical activity. Here, we show that supramolecular host-guest chemistry can be utilized as an alternative approach in nanoparticle functionalization and selective cell labeling. We have used cyclodextrin-conjugated quantum dots (QDs) for supramolecular host-guest interaction-based functionalization with folate (QD-folate) and riboflavin (QD-riboflavin), where cyclodextrin acts as a host for the folate/riboflavin guest. We demonstrate that QD-folate and QD-riboflavin selectively label cells that have over-expressed folate/riboflavin receptors and induce the endocytosis pathway similar to covalently conjugated folate-/riboflavin-based nanoprobes. However, labeling is highly sensitive to the molar ratio of folate/riboflavin to cyclodextrin and incubation time. The presented functionalization/labeling approach is unique as it does not require covalent conjugation and may be extended for in vivo ...Continue Reading

References

May 15, 1988·Analytical Biochemistry·M Wilchek, E A Bayer
Apr 1, 1997·The EMBO Journal·H L Monaco
Sep 3, 2002·Advanced Drug Delivery Reviews·Yingjuan Lu, Philip S Low
Nov 25, 2003·Analytical Chemistry·Brian M LingerfeltGeorge P Anderson
Nov 13, 2004·Angewandte Chemie·Eugenii Katz, Itamar Willner
Apr 14, 2005·Chemical Reviews·Nathaniel L Rosi, Chad A Mirkin
Jun 2, 2005·Nature Materials·Igor L MedintzHedi Mattoussi
Jun 21, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Joanna RejmanMassimo Conese
Jun 15, 2007·Molecular Pharmacology·Amy B ForakerPeter W Swaan
Jul 5, 2007·Nature Reviews. Molecular Cell Biology·Satyajit Mayor, Richard E Pagano
Jun 3, 2008·Advanced Drug Delivery Reviews·Igor I SlowingVictor S-Y Lin
Aug 21, 2008·Accounts of Chemical Research·Catherine J MurphySarah C Baxter
Mar 6, 2009·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Dalim Kumar RoyAsok K Mukherjee
Feb 4, 2010·Analytical Chemistry·Vincent E TurulaRasappa G Arumugham
Aug 4, 2010·Journal of the American Chemical Society·Arántzazu González-CampoAldrik H Velders
Nov 12, 2010·Physical Chemistry Chemical Physics : PCCP·Nikhil R Jana
Nov 25, 2011·Angewandte Chemie·Sarit S AgastiRalph Weissleder
Dec 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Haibao JinDeyue Yan
Apr 10, 2013·Pharmaceutical Research·Lisa M BarefordPeter W Swaan
May 16, 2013·Advanced Drug Delivery Reviews·Jianxiang Zhang, Peter X Ma
May 16, 2013·Nanoscale·Amit Ranjan MaityNikhil R Jana
May 2, 2014·Accounts of Chemical Research·Ruijiao DongXinyuan Zhu
Feb 25, 2015·Bioconjugate Chemistry·Regina BilanAlyona Sukhanova
Apr 11, 2015·Nature Reviews. Molecular Cell Biology·Ludger JohannesSatyajit Mayor
Jul 18, 2015·ACS Applied Materials & Interfaces·Claire SoumThomas Berthelot
Jan 2, 2016·The Journal of Physical Chemistry Letters·Atanu Chakraborty, Nikhil R Jana
Feb 27, 2016·Journal of the American Chemical Society·Min XueJames R Heath

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Citations

Feb 23, 2019·Frontiers in Pharmacology·Lisa BartmannZhuojun Wu
Feb 26, 2021·International Journal of Nanomedicine·Zehra EdisMunazza Ijaz
Nov 28, 2018·Analytical Chemistry·Xiaoni FangWenwan Zhong

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