Supramolecular solvent-based microextraction of ochratoxin A in raw wheat prior to liquid chromatography-fluorescence determination

Journal of Chromatography. a
Sergio García-FonsecaDolores Pérez-Bendito

Abstract

A supramolecular solvent made up of reverse micelles of decanoic acid, dispersed in a continuous phase of THF:water, was proposed for the simple, fast and efficient microextraction of OTA in wheat prior to liquid chromatography-fluorescence determination. The method involved the stirring of 300 mg-wheat subsamples (particle size 50microm) and 350microL of supramolecular solvent for 15min, subsequent centrifugation for 15min and the direct quantitation of OTA in the extract, previous 5.7-fold dilution with ethanol/water/acetic acid (49.5/49.5/1), against solvent-based calibration curves. No clean-up of the extracts or solvent evaporation was needed. Interactions between the supramolecular solvent and major matrix components in the wheat (i.e. carbohydrates, lipids and proteins) were investigated. The reverse micelles in the extractant induced gluten flocculation but only in the coacervation region of lower analytical interest (i.e. at percentages of THF above 11%). The quantitation of OTA was interference-free. Representativity of the 300 mg-wheat subsamples was proved by analysing a reference material. OTA recoveries in wheat ranged between 84% and 95% and the precision of the method, expressed as relative standard deviation, w...Continue Reading

References

Aug 27, 1998·Journal of Chromatography. a·M SolfrizzoA Visconti
May 20, 2008·Analytica Chimica Acta·Sergio García-FonsecaDolores Pérez-Bendito
May 20, 2008·Analytica Chimica Acta·Amalia García-PrietoDolores Pérez-Bendito
Jul 8, 2008·Journal of Chromatography. a·Ana Ballesteros-GómezDolores Pérez-Bendito

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Citations

Feb 19, 2013·Analytical and Bioanalytical Chemistry·Morteza MoradiHamid Asiabi
May 31, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·S A TittlemierR Krska
Jun 1, 2010·Toxins·Jorn C C Yu, Edward P C Lai
Jun 5, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maryam Ezoddin, Khosrou Abdi
Aug 14, 2012·Journal of Separation Science·Morteza Moradi, Yadollah Yamini
Dec 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sylwia BajkaczEwa Felis

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