Supramolecular Structure and Functional Analysis of the Type III Secretion System in Pseudomonas fluorescens 2P24

Frontiers in Plant Science
Ping LiuHai-Lei Wei

Abstract

The type III secretion system (T3SS) of plant and animal bacterial pathogens directs the secretion and injection of proteins into host cells. Some homologous genes of T3SS were found also in non-pathogenic bacteria, but the organization of its machinery and basic function are still unknown. In this study, we identified a T3SS gene cluster from the plant growth-promoting Pseudomonas fluorescens 2P24 and isolated the corresponding T3SS apparatus. The T3SS gene cluster of strain 2P24 is similar organizationally to that of pathogenic P. syringae, except that it lacks the regulator hrpR and the hrpK1 and hrpH genes, which are involved in translocation of proteins. Electron microscopy revealed that the T3SS supramolecular structure of strain 2P24 was comprised of two distinctive substructures: a long extracellular, filamentous pilus, and a membrane-embedded base. We show that strain 2P24 deploys a harpin homolog protein, RspZ1, to elicit a hypersensitive response when infiltrated into Nicotiana tabacum cv. xanthi leaves with protein that is partially purified, and by complementing the hrpZ1 mutation of pHIR11. The T3SS of strain 2P24 retained ability to secrete effectors, whereas its effector translocation activity appeared to be exc...Continue Reading

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Citations

Mar 12, 2020·Journal of Experimental Botany·Maged M SaadHeribert Hirt
Aug 28, 2020·International Journal of Molecular Sciences·Xiaoxiao ZhangTingchang Zhao
Aug 6, 2019·Frontiers in Microbiology·Ioannis A StringlisCorné M J Pieterse
Oct 9, 2021·Frontiers in Microbiology·Jing WangHai-Lei Wei

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Datasets Mentioned

BETA
KT582783

Methods Mentioned

BETA
PCR
density gradient centrifugation
electrophoresis

Software Mentioned

Genetics

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