PMID: 15378840Sep 24, 2004Paper

Suramin sensitizing cells to ionizing radiation by inactivating DNA-dependent protein kinase

Radiation Research
Yoshio HosoiNorio Suzuki

Abstract

Here we report that suramin sensitizes LM217, MDA-MB-468, T98G and A431 cells to ionizing radiation. Suramin sensitized cells to X radiation in a dose-dependent fashion, and longer exposure to suramin before X irradiation resulted in more efficient sensitization. The dose-modifying factors calculated from the survival curves were 1.18 in LM217 cells and 1.37 in MDA-MB-468 cells. Suramin did not sensitize Scid cells that had no DNA-dependent protein kinase activity. Suramin inhibited DNA-dependent protein kinase activity in vitro and in vivo. The concentration of suramin resulting in 50% inhibition in vitro was 1.7 microM in LM217 cells and 2.4 microM in MDA-MB-468 cells. Exposure of LM217 and MDA-MB-468 cells to suramin did not affect the level of Ku70 (G22P1) or Ku80 (XRCC5), but it increased the level of DNA-PKcs(PRKDC). Suramin did not sensitize LM217 or MDA-MB-468 cells to UV radiation. Suramin's effects were not caused by accumulation of cells in a specific phase of the cell cycle. These results suggest that suramin sensitizes cells to ionizing radiation by inhibiting DNA-dependent protein kinase activity.

References

Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K BojanowskiA K Larsen
Jun 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C MyersM M Uhrich
Feb 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·K A BiedermannJ M Brown
Jan 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S OlivierG Milano
Aug 15, 1990·Journal of the National Cancer Institute·M Pollak, M Richard
Feb 1, 1986·Molecular and Cellular Biology·J P Murnane
Jul 1, 1987·Journal of Cellular Physiology·R J CoffeyH L Moses
Nov 1, 1995·Nature Medicine·C A Stein
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·N J FinnieS P Jackson
Aug 1, 1994·Annals of Neurology·J W RussellJ L Podratz
Dec 1, 1993·Cellular and Molecular Neurobiology·K TsutsumiS Shibata
Mar 1, 1996·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·S BaghdiguianJ Fantini
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·O Hammarsten, G Chu
May 5, 1998·Cancer Letters·A R GagliardiD C Collins
Dec 5, 1998·Journal of Molecular Biology·C Y ChiuP L Stewart
Feb 16, 1999·International Journal of Radiation Oncology, Biology, Physics·A G PolischoukR Lewensohn
Mar 11, 1999·Cancer Chemotherapy and Pharmacology·A LokshinM L Levitt
Feb 13, 2001·International Journal of Radiation Oncology, Biology, Physics·K SakataM Hareyama
Jul 9, 2002·Investigational New Drugs·Maninderjeet KaurWilliam D Figg

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Citations

May 7, 2011·World Journal of Gastroenterology : WJG·Jia-Qi LiXue-Rong Wang

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