PMID: 9451451Feb 6, 1998Paper

Surface activity properties of cysteine-substituted C-terminal melittin analogues

Biochimie
S C EbelingD Sheehan

Abstract

In order to extend our knowledge of factors important in the surface activity of melittin, cysteine was substituted for lysine-21 and lysine-21/glutamine-25 in a pair of synthetic peptide analogues. The first of these changes resulted in only modest effects on secondary structure (determined in 50% trifluoroethanol), emulsification and surface tension properties. Introduction of a second cysteine greatly reduced both the rate of surface tension decay and the equilibrium surface tension attained, although secondary structure (determined in 50% trifluoroethanol) was only slightly affected by this modification. This latter peptide completely lacked emulsification and haemolytic properties and was found to oligomerise readily due to the formation of intermolecular, disulphide bridges. These results indicate that oligomerisation abolishes surface activity in melittin.

References

Jun 1, 1979·FEBS Letters·J C TalbotC Lussan
Sep 18, 1979·Biochemistry·E KnöppelW Wickner
Dec 22, 1992·Biochemistry·S E Blondelle, R A Houghten
Nov 1, 1992·International Journal of Peptide and Protein Research·D WadeR B Merrifield
Sep 30, 1991·Biochimica Et Biophysica Acta·G Beschiaschvili, H D Baeuerle
Apr 9, 1991·FEBS Letters·C E DempseyI D Campbell
May 7, 1990·Biochimica Et Biophysica Acta·C E Dempsey
Mar 1, 1990·International Journal of Peptide and Protein Research·G B Fields, R L Noble
Apr 5, 1988·European Journal of Biochemistry·R BazzoI D Campbell
Jul 28, 1972·Science·E Habermann
Feb 1, 1970·Analytical Biochemistry·S Ward
Feb 1, 1983·Biochimica Et Biophysica Acta·R C HiderA S Tatham
Mar 1, 1981·Infection and Immunity·F S Nolte, F A Kapral
Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner
Apr 1, 1995·International Journal of Peptide and Protein Research·L ZhongA G Rao
May 4, 1995·Biochimica Et Biophysica Acta·T Benachir, M Lafleur
Mar 21, 1995·Biochemistry·K J BakerA G Lee
Oct 6, 1993·Biochimica Et Biophysica Acta·S E BlondelleR A Houghten
May 7, 1993·Biochimica Et Biophysica Acta·J A WerkmeisterD E Rivett
Jun 1, 1996·The Biochemical Journal·D E RivettJ A Werkmeister
Feb 1, 1996·Trends in Biotechnology·S E Blondelle, R A Houghten

❮ Previous
Next ❯

Citations

Feb 19, 2004·Journal of Agricultural and Food Chemistry·Shuryo NakaiAlan Paulson

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.