Surface adsorption alters the susceptibility of whey proteins to pepsin-digestion

Journal of Colloid and Interface Science
Amir Malaki NikMilena Corredig

Abstract

An in vitro digestion model mimicking the gastric phase of the human gastrointestinal tract coupled with SDS-PAGE and MALDI-TOF mass spectroscopy was employed to study the hydrolysis profiles of whey proteins in solution and adsorbed at the oil-water interface. The objective of this work was to understand the differences in hydrolysis behaviour of whey protein isolates once adsorbed at the interface, and comparisons were carried out with pure beta-lactoglobulin and alpha-lactalbumin fractions. In solution, while beta-lactoglobulin appeared to be resistant to enzymatic treatment, alpha-lactalbumin was fully degraded. Adsorption of both proteins at the oil-water interface affected their conformational structure and susceptibility to peptic hydrolysis. Adsorbed beta-lactoglobulin was hydrolyzed into small polypeptides and in contrast, the resistance of alpha-lactalbumin to pepsin increased upon adsorption at the interface. In addition, changes in the particle size distribution of the droplets during pepsin hydrolysis mainly depended on the original protein concentration. The results suggested that exchanges occur at the interface between adsorbed and non-adsorbed protein, that is to say that either some protein desorb from the int...Continue Reading

References

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Citations

Feb 16, 2013·Journal of Agricultural and Food Chemistry·Nicoleta StănciucMarc Hendrickx
Jul 22, 2011·Food & Function·Amir Malaki NikMilena Corredig
Nov 2, 2014·Soft Matter·Teresa del Castillo-SantaellaJulia Maldonado-Valderrama
Jun 27, 2013·Food & Function·Raphaela Araujo MantovaniRosiane Lopes Cunha
Jul 20, 2011·Journal of the Science of Food and Agriculture·Victor J MorrisAllan P Gunning
Mar 8, 2011·Advances in Colloid and Interface Science·P J Wilde, B S Chu
Apr 18, 2016·Biochimica Et Biophysica Acta·Mauro MarengoAlberto Barbiroli
Dec 4, 2014·Food Chemistry·Saehun MunDavid Julian McClements
Jan 5, 2011·Colloids and Surfaces. B, Biointerfaces·Amir Malaki NikMilena Corredig
Nov 23, 2011·Food & Function·David Julian McClements, Hang Xiao
Jul 17, 2014·Biomacromolecules·N ScheubleP Fischer
Dec 29, 2018·Journal of Dairy Science·Carlotta GirominiAntonella Baldi
Feb 8, 2018·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·I ProdicT Cirkovic Velickovic
Sep 29, 2020·Journal of Agricultural and Food Chemistry·Angelique VandemoorteleBruno De Meulenaer

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