PMID: 6969855Jan 1, 1981Paper

Surface antigen differentiation during human myogenesis in culture

Nature
F S Walsh, M A Ritter

Abstract

The fusion of mononucleate myoblast cells into multinucleate myotubes (myogenesis) has often been studied as a model system of terminal cellular differentiation. Although cell-surface changes during myogenesis have attracted much attention and a variety of surface antigens including the nicotinic cholinergic receptor, acetylcholinesterase and muscle lectins have been shown to be present on muscle cells, a detailed identification of markers specific to the various cell types has not been attempted. This is mainly because fibroblasts are a major contaminating cell type in primary muscle cultures and these cells have no known distinctive cell-surface antigen(s). For instance, surface fibronectin has been used to distinguish fibroblasts in the rat peripheral nervous system in vitro but has proved ineffective in the human muscle cell system. In addition, Lesley and Lennon found that Thy-1 antigen was present on myoblasts but not myotubes of the rat L6 muscle cell line and primary cultures. However, Thy-1 antigen is also present on rat fibroblasts. Thus, unequivocal identification of the major mononucleate cell types in muscle cultures, fibroblasts and myoblasts has not been possible. We report here the use of two surface antigens, T...Continue Reading

References

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Citations

Jan 12, 1999·Muscle & Nerve·J R BlantonC A Bidwell
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·B E PetersenG K Michalopoulos
May 1, 1993·Muscle & Nerve·A BaroffioC R Bader
Mar 1, 1982·Die Naturwissenschaften·U Wintersberger
Jan 1, 1993·Cytotechnology·L MetzingerA C Passaquin
Sep 6, 2007·In Vitro Cellular & Developmental Biology. Animal·Michele L MarquetteMarguerite Sognier
Mar 25, 1982·Brain Research·J T KemsheadM F Greaves
Jun 1, 1983·Developmental Biology·M F Marusich, S B Simpson
Jul 1, 1985·Developmental Biology·S J Kaufman, R F Foster
Sep 1, 1984·Experimental Cell Research·C A GrahamN C Nevin
Mar 1, 1983·Journal of the Neurological Sciences·R YasinE J Thompson
Oct 1, 1992·Journal of the Neurological Sciences·L AustinN Vakakis
Apr 10, 2007·Nature Medicine·Tiziano BarberiLorenz Studer
Aug 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·C A QuinnF S Walsh
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·W J RettigL J Old
Jan 1, 1986·The Journal of Cell Biology·G K Pavlath, H M Blau
Jan 1, 1988·Journal of Neurology, Neurosurgery, and Psychiatry·F S WalshJ G Dickson
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fan ZhangHelen M Blau
Oct 27, 2007·Pediatric Surgery International·Nathalie Hirt-BurriJudith Hohlfeld
Feb 28, 2003·In Vitro Cellular & Developmental Biology. Animal·J MichalK M Byrne
Dec 31, 2015·Journal of Muscle Research and Cell Motility·Barbara ŚwierczekKarolina Archacka
May 22, 2009·Experimental Gerontology·Tiziana PietrangeloStefania Fulle
Mar 16, 2016·Biochemical and Biophysical Research Communications·Ester Sara Di FilippoStefania Fulle
May 4, 2004·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Toshitaka HoppoIwao Ikai

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