Surface antigens contribute differently to the pathophysiological features in serotype K1 and K2 Klebsiella pneumoniae strains isolated from liver abscesses

Gut Pathogens
Kuo-Ming YehLeung-Kei Siu

Abstract

The virulence role of surface antigens in a single serotype of Klebsiella pneumoniae strain have been studied, but little is known about whether their contribution will vary with serotype. To investigate the role of K and O antigen in hyper-virulent strains, we constructed O and K antigen deficient mutants from serotype K1 STL43 and K2 TSGH strains from patients with liver abscess, and characterized their virulence in according to the abscess formation and resistance to neutrophil phagocytosis, serum, and bacterial clearance in liver. Both of K1 and K2-antigen mutants lost their wildtype resistance to neutrophil phagocytosis and hepatic clearance, and failed to cause abscess formation. K2-antigen mutant became serum susceptible while K1-antigen mutant maintained its resistance to serum killing. The amount of glucuronic acid, indicating the amount of capsular polysaccharide (CPS, K antigen), was inversed proportional to the rate of phagocytosis. O-antigen mutant of serotype K1 strains had significantly more amount of CPS, and more resistant to neutrophil phagocytosis than its wildtype counterpart. O-antigen mutants of serotype K1 and K2 strains lost their wildtype serum resistance, and kept resistant to neutrophil phagocytosis. ...Continue Reading

References

Aug 1, 1976·Carbohydrate Research·C ErbingJ Lönngren
Nov 1, 1992·Microbial Pathogenesis·R PodschunU Ullmann
Mar 1, 1986·Journal of Medical Microbiology·A M Simoons-SmitD M MacLaren
Oct 1, 1986·Archives of Internal Medicine·Y C LiuC L Lin
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Apr 14, 1999·Molecular Microbiology·C Whitfield, I S Roberts
Feb 12, 2000·Journal of Medical Microbiology·R PodschunU Ullman
Mar 12, 2003·FEMS Immunology and Medical Microbiology·Philipp M LepperMatthias Trautmann
Feb 24, 2004·Infection and Immunity·Sunita Shankar-SinhaJohn G Younger
Feb 3, 2006·The Journal of Infectious Diseases·Yi-Ping ChuangJin-Town Wang
Nov 16, 2006·Liver International : Official Journal of the International Association for the Study of the Liver·Manfred BilzerAlexander L Gerbes
Apr 6, 2011·Laboratory Investigation; a Journal of Technical Methods and Pathology·Chang-Phone FungL Kristopher Siu

❮ Previous
Next ❯

Citations

Jan 25, 2017·Antimicrobial Agents and Chemotherapy·Frank R DeLeoBarry N Kreiswirth
Nov 12, 2018·Cellular Microbiology·Michael Dominic ChuaJulian Andrew Guttman
Apr 25, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yang LiuWei Zhang
Jan 30, 2021·Pathogens and Disease·Sebastian BruchmannFrancesca L Short
Jul 21, 2017·Chemical Reviews·Mingying YangChuanbin Mao

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
glucuronic acid assay

Software Mentioned

SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.