PMID: 3765354Jun 1, 1986Paper

Surface antigens of virulent strains of Aeromonas hydrophila

Veterinary Immunology and Immunopathology
J S DooleyT J Trust

Abstract

Antiserum was raised in rabbits to whole cells of a representative strain from a group of A. hydrophila strains exhibiting enhanced virulence for fish. The major surface antigens of the strain were identified by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. The lipopolysaccharide (LPS) was examined using SDS-PAGE and silver staining. It was found to possess O polysaccharide chains of homogeneous length that were highly immunogenic. The LPS was conserved both morphologically and antigenically throughout the high virulence group. Heat-labile protein antigens were detected after absorption of the antiserum with boiled cells of the homologous strain. Only one major protein antigen, with a molecular weight of approximately 52,000, was present in outer membrane preparations or in whole cell lysates. A representative strain from the high virulence group, strain TF7, was shown by electron microscopy to be covered by a regular surface protein array (S-layer) which was found to be composed primarily of the 52 KD protein antigen. All the other members of the A. hydrophila high virulence group were shown to possess similar S-layers.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Sep 1, 1974·Canadian Journal of Microbiology·T J Trust, R A Sparrow
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Dec 1, 1980·Canadian Journal of Microbiology·K R MittalR Lallier

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Citations

Jan 1, 1989·Critical Reviews in Microbiology·M Altwegg, H K Geiss
Jan 1, 1996·Progress in Biophysics and Molecular Biology·M Sára, U B Sleytr
Sep 1, 1990·Journal of Bacteriology·J D DubreuilT J Trust
May 1, 1989·Journal of Clinical Microbiology·S A PalumboR L Buchanan
Oct 1, 1988·Infection and Immunity·S J PaulaR Sakazaki

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