Surface changes induced by osmotic shrinkage on large unilamellar vesicles

Chemistry and Physics of Lipids
E A DisalvoE A Lissi

Abstract

Osmotic shrinkage changes the surface properties of dipalmitoylphosphatidylcholine large unilamellar vesicles depending on the phase state of the bilayer. In the gel state, shrinkage produces an increase in the adsorption of hydrophobic dyes, such as Merocyanine 540 (MC540) monomers, toluidine and anilinonaphthalene sulfonic acid (TNS, ANS). In the fluid state, shrinkage does not affect the bilayer surface when gradients between the inner and the outer compartments below 0.2-0.25 M NaCl (higher concentration outside) are applied. Larger differences in concentrations produce an increase in packing as inferred from the desorption of the MC monomers. Kinetic experiments show that the surface changes correlate with the volume decrease produced by the water extrusion from the vesicle interior. It is interpreted that the decrease of water content compels the vesicles to a state in which defects at the membrane surface are likely to occur when the bilayer is in the gel state.

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Citations

Jul 1, 1988·Advances in Colloid and Interface Science·E A Disalvo
May 28, 2011·Colloids and Surfaces. B, Biointerfaces·Paolo De MariaCarla Gasbarri
Dec 19, 2002·Biochimica Et Biophysica Acta·Anne-Laure BernardJean-Marc di Meglio
Nov 1, 2005·Archives of Biochemistry and Biophysics·E Elizabeth TymczyszynE Anibal Disalvo
May 10, 2015·Chemistry and Physics of Lipids·M AhumadaE A Lissi
Mar 2, 1999·Archives of Biochemistry and Biophysics·M C LuzardoE A Disalvo
Jul 17, 2012·Chemistry and Physics of Lipids·Elsa AbuinManuel Ahumada
Nov 5, 2016·Colloids and Surfaces. B, Biointerfaces·M A PalominosM A Soto-Arriaza
Nov 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Dana R AdamsRobert Langer
Jan 17, 2004·Archives of Biochemistry and Biophysics·M Cecilia MachadoEmilio A Rivas
Jul 17, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Judith U De MelGerald J Schneider

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