Surface characteristics of Pseudomonas aeruginosa grown in a chamber implant model in mice and rats.

Infection and Immunity
N M KellyR E Hancock

Abstract

Pseudomonas aeruginosa PAO1 was grown in vivo in chambers implanted into the peritoneums of mice and rats. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of extracts of bacterial cells taken from the chambers and washed to remove loosely bound host proteins revealed the presence of the major outer membrane proteins D2, E, F, G, and H2. Western immunoblotting with specific antisera confirmed the presence of porin protein F and lipoprotein H2. However, there was no apparent induction of the phosphate starvation-inducible porin P or the divalent cation starvation-inducible protein H1. Small amounts of proteins with molecular weights similar to those of the iron-regulated outer membrane proteins were found in cells grown in vivo; however, their presence could not be confirmed immunologically. The presence of pili and flagella on the cells grown in vivo was demonstrated by electron microscopy and Western immunoblotting. A consistent alteration in the lipopolysaccharide banding pattern was observed after growth in vivo. Compared with cells of strain PAO1 grown in vitro, cells grown in vivo appeared to lack a series of high-molecular-weight O-antigen-containing lipopolysaccharide bands and gained a new series of lower-molec...Continue Reading

References

Dec 1, 1979·Journal of Bacteriology·R E Hancock, A M Carey
Mar 1, 1986·Molecular & General Genetics : MGG·K Poole, R E Hancock
Jul 1, 1985·Infection and Immunity·W EngelsC P van Boven
Jan 1, 1982·Analytical Biochemistry·C M Tsai, C E Frasch
Dec 1, 1982·The Journal of Infectious Diseases·L M MuthariaR E Hancock
Mar 1, 1983·Infection and Immunity·S J CryzR Germanier
May 1, 1984·Infection and Immunity·S J CryzR Germanier
Jan 1, 1981·Journal of Bacteriology·R E HancockA M Carey
Jan 1, 1982·Infection and Immunity·I A HolderT C Montie

❮ Previous
Next ❯

Citations

Dec 1, 1990·FEMS Microbiology Immunology·A D SutherlandI R Poxton
Oct 1, 1992·Antimicrobial Agents and Chemotherapy·N RousseauF Malouin
Mar 1, 1989·Infection and Immunity·J L KluftingerR E Hancock

❮ Previous
Next ❯

Related Concepts

Related Feeds

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.