PMID: 3946407Mar 1, 1986

Surface charge and hydrophobic properties of fresh and cryopreserved blood phagocytes as determined by partition in two-phase aqueous polymer systems

American Journal of Hematology
R S HillD E Brooks

Abstract

Cell partitioning in two-phase aqueous polymer systems was used to examine hydrophobic and surface charge-related membrane properties of fresh and cryopreserved human blood phagocytes. This technique is highly sensitive to cell surface characteristics, and the partition behavior depends exponentially on the membrane properties involved. The transition from fresh to cryopreserved and reconstituted cells was accompanied by a significant loss of net negative charge without detectable alteration in hydrophobic membrane properties as detected by the partition technique. The partition coefficient (PC), which is the proportion of cells partitioning into the upper phase, when measured for fresh cells mixed with dimethyl sulfoxide (Me2SO) and cryopreserved leukocytes. No difference was detected between the PCs of the total leukocytes and phagocytes as determined by differential leukocyte counts of the upper polymer phase. The significantly reduced PC of cells prepared by dextran (Dx) separation in both charge-sensitive and -insensitive systems is attributable to the capacity of Dx to adsorb, in part irreversibly, to cells so that those carrying Dx tend to partition with Dx in the lower phase. These results serve to illustrate the utilit...Continue Reading

References

Sep 1, 1976·British Journal of Haematology·R M LowenthalK H Th'ng
Feb 16, 1976·Molecular and Cellular Biochemistry·E ErikssonG Johansson
Feb 28, 1973·Biochimica Et Biophysica Acta·R ReithermanS H Barondes
Apr 19, 1980·Lancet·R S Hill, C A Mackinder
Jan 1, 1984·British Journal of Haematology·D M McCarthyJ M Goldman

Citations

Jan 1, 1990·Transfusion Medicine Reviews·T A Lane
Sep 1, 1987·Family Process·E H Auerswald

Related Concepts

Blood Preservation
Cell Separation
Melting
Phagocytes
Vigilon
Polymers

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