Surface Charge Modulates Protein-Protein Interactions in Physiologically Relevant Environments

Biochemistry
Alex J GusemanGary J Pielak

Abstract

Protein-protein interactions are fundamental to biology yet are rarely studied under physiologically relevant conditions where the concentration of macromolecules can exceed 300 g/L. These high concentrations cause cosolute-complex contacts that are absent in dilute buffer. Understanding such interactions is important because they organize the cellular interior. We used 19F nuclear magnetic resonance, the dimer-forming A34F variant of the model protein GB1, and the cosolutes bovine serum albumin (BSA) and lysozyme to assess the effects of repulsive and attractive charge-charge dimer-cosolute interactions on dimer stability. The interactions were also manipulated via charge-change variants and by changing the pH. Charge-charge repulsions between BSA and GB1 stabilize the dimer, and the effects of lysozyme indicate a role for attractive interactions. The data show that chemical interactions can regulate the strength of protein-protein interactions under physiologically relevant crowded conditions and suggest a mechanism for tuning the equilibrium thermodynamics of protein-protein interactions in cells.

References

Jan 1, 1987·Annual Review of Biochemistry·P A Srere
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·E H McConkey
Feb 27, 1999·Current Opinion in Structural Biology·R J Ellis, F U Hartl
Oct 9, 2001·Trends in Biochemical Sciences·R J Ellis
Dec 19, 2003·Nucleic Acids Research·K Abdulla BavaAkinori Sarai
Dec 20, 2003·Nature·Christopher M Dobson
Jul 5, 2005·Current Opinion in Structural Biology·Daniel P Ryan, Jacqueline M Matthews
Dec 14, 2007·Proteins·JunGoo JeeAngela M Gronenborn
Feb 9, 2008·Chembiochem : a European Journal of Chemical Biology·Peter B CrowleyJimmy Muldoon
Aug 5, 2009·Biophysical Journal·Yael PhillipGideon Schreiber
Mar 31, 2011·Chembiochem : a European Journal of Chemical Biology·Peter B CrowleyTatiana Papkovskaia
Sep 8, 2012·Journal of the American Chemical Society·Yaqiang WangGary J Pielak
Nov 22, 2012·Biochemistry·Laura A BentonGary J Pielak
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mohona SarkarGary J Pielak
Feb 21, 2014·Biochemistry·Mohona SarkarGary J Pielak
Jun 4, 2014·Journal of the American Chemical Society·Michael SenskeSimon Ebbinghaus
Jun 6, 2014·Chemical Reviews·Francois-Xavier TheilletPhilipp Selenko
Dec 6, 2014·Current Opinion in Structural Biology·Austin E SmithConggang Li
Jan 6, 2015·Angewandte Chemie·David GnuttSimon Ebbinghaus
Jan 28, 2015·Proceedings of the National Academy of Sciences of the United States of America·William B MonteithGary J Pielak
Sep 24, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jens DanielssonMikael Oliveberg
Jan 13, 2016·Proceedings of the National Academy of Sciences of the United States of America·Austin E SmithGary J Pielak
Oct 30, 2016·Trends in Biochemical Sciences·Germán Rivas, Allen P Minton
Sep 28, 2016·Journal of the American Chemical Society·Rachel D Cohen, Gary J Pielak
Nov 5, 2016·Protein Science : a Publication of the Protein Society·Ciara KynePeter B Crowley
Dec 16, 2016·Protein Science : a Publication of the Protein Society·Rachel D Cohen, Gary J Pielak
Jan 20, 2017·Biochemistry·Alex J Guseman, Gary J Pielak
Jun 1, 2013·Biophysics Reviews·Mohona SarkarGary J Pielak
Jun 2, 2017·Protein Science : a Publication of the Protein Society·Rachel D Cohen, Gary J Pielak
Jun 14, 2017·Proceedings of the National Academy of Sciences of the United States of America·Shahar SukenikMartin Gruebele
Aug 24, 2017·Biochemistry·Ciara Kyne, Peter B Crowley

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Citations

Oct 12, 2018·Proceedings of the National Academy of Sciences of the United States of America·Alex J GusemanGary J Pielak
May 28, 2020·Physical Chemistry Chemical Physics : PCCP·Soundhararajan Gopi, Athi N Naganathan
Aug 14, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yin YangDaniella Goldfarb
Dec 7, 2018·Chembiochem : a European Journal of Chemical Biology·Birgit Köhn, Michael Kovermann
Jan 24, 2021·Advanced Drug Delivery Reviews·Bhushan N KharbikarTejal A Desai
Nov 5, 2019·Journal of Pharmaceutical Sciences·Thomas M Laue, Steven J Shire
Jan 14, 2021·Analytical Chemistry·Yunfei HuMaili Liu
Mar 4, 2021·American Journal of Physiology. Cell Physiology·Dimitrios I Zeugolis
Dec 3, 2020·Structure·Alex J GusemanAngela M Gronenborn
Mar 5, 2021·Current Opinion in Structural Biology·Martin Gruebele, Gary J Pielak
Dec 8, 2020·Current Opinion in Structural Biology·Samantha S Stadmiller, Gary J Pielak
Mar 16, 2021·Communications Chemistry·Rita PuglisiPiero A Temussi
Apr 17, 2021·Journal of Biomaterials Science. Polymer Edition·Xiangyu WangJunyu Liu
Apr 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Shannon L SpeerConggang Li
May 1, 2021·Cells·Sergio Garnica-GalvezDimitrios I Zeugolis
Apr 22, 2020·Current Research in Structural Biology·Sarah LeebJens Danielsson
Jul 26, 2018·Journal of the American Chemical Society·Shahar SukenikMartin Gruebele
Jan 25, 2020·Biochemistry·I-Te ChuGary J Pielak
May 3, 2019·The Journal of Physical Chemistry. B·Yansheng YeConggang Li
Sep 17, 2020·The Journal of Physical Chemistry. B·Samantha S StadmillerGary J Pielak
Aug 15, 2021·Chembiochem : a European Journal of Chemical Biology·Hannah WelteMichael Kovermann

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