Surface chemical immobilization of bioactive peptides on synthetic polymers for cardiac tissue engineering

Journal of Biomaterials Science. Polymer Edition
Elisabetta RoselliniNiccoletta Barbani

Abstract

The aim of this work was the development of new synthetic polymeric systems, functionalized by surface chemical modification with bioactive peptides, for myocardial tissue engineering. Polycaprolactone and a poly(ester-ether-ester) block copolymer synthesized in our lab, polycaprolactone-poly(ethylene oxide)-polycaprolactone (PCL-PEO-PCL), were used as the substrates to be modified. Two pentapeptides, H-Gly-Arg-Gly-Asp-Ser-OH (GRGDS) from fibronectin and H-Tyr-Ile-Gly-Ser-Arg-OH (YIGSR) from laminin, were used for the functionalization. Polymeric membranes were obtained by casting from solutions and then functionalized by means of alkaline hydrolysis and subsequent coupling of the bioactive molecules through 1-(3-dimethylaminopropyl)-3-ethylcarbodimide hydrochloride/N-hydroxysuccinimide chemistry. The hydrolysis conditions, in terms of hydrolysis time, temperature, and sodium hydroxide concentration, were optimized for the two materials. The occurrence of the coupling reaction was demonstrated by infrared spectroscopy, as the presence on the functionalized materials of the absorption peaks typical of the two peptides. The peptide surface density was determined by chromatographic analysis and the distribution was studied by infr...Continue Reading

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Citations

Dec 5, 2015·Biomacromolecules·Trevor R HamJustin M Saul
Sep 2, 2015·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kunal J Rambhia, Peter X Ma
May 17, 2018·Biomaterials Science·Mahboubeh JafarkhaniMasoud Mozafari
Nov 7, 2019·Journal of Biomaterials Applications·Caterina FratiElisabetta Rosellini
Sep 4, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Xianbao LiuJian'an Wang

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infrared spectroscopy

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