PMID: 8457577Mar 30, 1993Paper

Surface chemistry of binary mixtures of phospholipids in monolayers. Infrared studies of surface composition at varying surface pressures in a pulmonary surfactant model system

Biochemistry
F R RanaR A Dluhy

Abstract

Phospholipid monomolecular films at the air/water interface were studied using Langmuir-Blodgett (L-B) surface chemistry, 31P NMR spectroscopy, and infrared (IR) spectroscopy. These monolayers were composed of binary mixtures of acyl chain perdeuterated 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (i.e., DPPC-d62) with 1,2-dipalmitoyl-sn-glycero-3-phosphoglycerol (i.e., DPPG). This particular PC-PG binary mixture was chosen for study since this lipid system has been used as a model for pulmonary surfactant, especially in conjunction with the so-called "squeezing-out" hypothesis of pulmonary mechanics. This theory predicts that upon successive compression-expansion cycles, a surfactant surface film will reorganize to exclude all components except DPPC, thus resulting in a stable, low surface tension film. Several general results were obtained from these experiments. First, we have developed a combined spectroscopic assay using high-resolution 31P NMR spectroscopy in combination with the C-H and C-D vibrational intensities obtained from the IR spectroscopy of binary mixtures in which one component is acyl chain perdeuterated. Using attenuated total reflectance IR spectroscopy of transferred L-B films, this combined spectroscopic a...Continue Reading

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Citations

Nov 17, 1995·Chemistry and Physics of Lipids·R W Evans
May 12, 2001·Respiration Physiology·R Banerjee, J R Bellare
Jan 18, 2008·Biophysical Journal·Susan L Duncan, Ronald G Larson
Sep 28, 2014·Chemistry and Physics of Lipids·Elisa Parra, Jesús Pérez-Gil
May 8, 1999·Journal of Applied Physiology·E P IngenitoM Johnson
Oct 20, 2001·Journal of Applied Physiology·L M ConnorE M Postlethwait
Jul 4, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Lu Xu, Yi Y Zuo

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