Surface coat material associated with the cells of the developing lens vesicle in the chick embryo

The Anatomical Record
J J Van Rybroek, M D Olson

Abstract

Ruthenium red (RR) and cetylpyridinium chloride (CPC) were used to demonstrate the distribution of cell surface coat material (SCM) on the free epithelial surface of the developing lens vesicle in stages 14-17 (50-64 hours) chick embryos. Observations were made by light microscopy and transmission. (TEM) and scanning (SEM) electron microscopy. A progressive increase in SCM is observed on cellular apices within the epithelium of the lens vesicle by means of RR staining, particularly at the margins of the aperture which are the sites of presumptive fusion. In contrast, a relatively thin layer of SCM persist on the adjacent surface ectoderm. Ruthenium red-positive SCM extends across the aperture of the lens vesicle prior to initial contact between the advancing epithelial surfaces. The presence of abundant SCM is interpreted as a possible significant prerequisite to invagination and to epithelial adhesion and fusion prior to detachment of the lens from surface ectoderm. When CPC is added to the fixative, a flocculent precipitate over the aperture of the lens vesicle and an associated band of modified surface ectoderm which extends ventrally from its lower margin are observed. The modified ectoderm and associated SCM likely represe...Continue Reading

References

Jan 1, 1976·International Review of Cytology·J H Luft
Aug 1, 1978·Developmental Biology·L L Mak
Jan 1, 1977·The American Journal of Anatomy·L L Litke, F N Low
Apr 1, 1979·The Anatomical Record·J A García-PorreroJ L Ojeda
Aug 9, 1977·Anatomy and Embryology·M Bancroft, R Bellairs
Nov 1, 1977·The American Journal of Anatomy·J D Gaare, J Langman
Jun 30, 1975·Annals of the New York Academy of Sciences·B Burnside
Jun 30, 1975·Annals of the New York Academy of Sciences·J Piatigorsky
Oct 1, 1965·Histochemie. Histochemistry. Histochimie·J E Scott, J Dorling
Jan 1, 1974·International Review of Cytology·P Karfunkel
Sep 15, 1972·Science·N Sharon, H Lis
Jul 1, 1969·The Journal of Experimental Zoology·J T Wrenn, N K Wessells
Jan 1, 1971·Experimental Cell Research·W Bernhard, S Avrameas
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·B BYERS, K R PORTER
Jan 1, 1951·Journal of Morphology·V HAMBURGER, H L HAMILTON

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Citations

Jan 1, 1984·Bulletin of Mathematical Biology·J S Clowes, G D Wassermann
Oct 1, 1983·Experimental Eye Research·M IrelandH Maisel
Feb 1, 1988·The Anatomical Record·A R Sinning, M D Olson
Oct 12, 2002·Developmental Biology·A GatoC Martínez-Alvarez

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