Surface dependence of protein nanocrystal formation

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Aitziber Eleta LópezJosé L Toca-Herrera

Abstract

The self-assembly kinetics and nanocrystal formation of the bacterial surface-layer-protein SbpA are studied with a combination of quartz crystal microbalance with dissipation monitoring (QCM-D) and atomic force microscopy (AFM). Silane coupling agents, aminopropyltriethoxysilane (APTS) and octadecyltrichlorosilane (OTS), are used to vary the protein-surface interaction in order to induce new recrystallization pathways. The results show that the final S-layer crystal lattice parameters (a = b = 14 nm, gamma = 90 degrees ), the layer thickness (15 nm), and the adsorbed mass density (1700 ng cm(-2)) are independent of the surface chemistry. Nevertheless, the adsorption rate is five times faster on APTS and OTS than on SiO(2,) strongly affecting protein nucleation and growth. As a consequence, protein crystalline domains of 0.02 microm(2) for APTS and 0.05 microm(2) for OTS are formed, while for silicon dioxide the protein domains have a typical size of about 32 microm(2). In addition, more-rigid crystalline protein layers are formed on hydrophobic substrates. In situ AFM experiments reveal three different kinetic steps: adsorption, self-assembly, and crystalline-domain reorganization. These steps are corroborated by frequency-dis...Continue Reading

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Citations

May 31, 2011·Physical Chemistry Chemical Physics : PCCP·Aitziber Eleta LópezJosé L Toca-Herrera
Jan 29, 2013·International Journal of Molecular Sciences·Dietmar PumUwe B Sleytr
Oct 5, 2013·The Journal of Chemical Physics·Ainhoa LejardiJosé L Toca-Herrera
Mar 6, 2013·Calcified Tissue International·James J De YoreoMichael H Nielsen
Aug 18, 2012·Journal of Molecular Recognition : JMR·Robert E Speight, Matthew A Cooper
May 8, 2016·Analytica Chimica Acta·Estefanía Moreno-GordalizaThomas Hankemeier
Feb 4, 2014·FEMS Microbiology Reviews·Uwe B SleytrDietmar Pum
Jul 24, 2010·Journal of Structural Biology·Birgit KainzJosé L Toca-Herrera
Mar 31, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Leopold J GrunerGerhard Rödel
Oct 8, 2017·Microscopy Research and Technique·Maria SumarokovaJosé L Toca-Herrera
Oct 9, 2018·Microscopy Research and Technique·Alberto Moreno-CencerradoJosé L Toca-Herrera
Feb 6, 2019·Materials·Vicente J BolosJose L Toca-Herrera
Feb 2, 2017·Beilstein Journal of Nanotechnology·Jagoba IturriJosé Luis Toca-Herrera
Apr 8, 2020·Science China. Life Sciences·Feng LiXian-En Zhan
Feb 22, 2017·International Journal of Molecular Sciences·Andreas BreitwieserDietmar Pum

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