Surface Display of Small Affinity Proteins on Synechocystis sp. Strain PCC 6803 Mediated by Fusion to the Major Type IV Pilin PilA1

Journal of Bacteriology
Ivana CengicElton P Hudson

Abstract

Functional surface display of small affinity proteins, namely, affibodies (6.5 kDa), was evaluated for the model cyanobacterium Synechocystis sp. strain PCC 6803 through anchoring to native surface structures. These structures included confirmed or putative subunits of the type IV pili, the S-layer protein, and the heterologous Escherichia coli autotransporter antigen 43 system. The most stable display system was determined to be through C-terminal fusion to PilA1, the major type IV pilus subunit in Synechocystis, in a strain unable to retract these pili (ΔpilT1). Type IV pilus synthesis was upheld, albeit reduced, when fusion proteins were incorporated. However, pilus-mediated functions, such as motility and transformational competency, were negatively affected. Display of affibodies on Synechocystis and the complementary anti-idiotypic affibodies on E. coli or Staphylococcus carnosus was able to mediate interspecies cell-cell binding by affibody complex formation. The same strategy, however, was not able to drive cell-cell binding and aggregation of Synechocystis-only mixtures. Successful affibody tagging of the putative minor pilin PilA4 showed that it locates to the type IV pili in Synechocystis and that its extracellular a...Continue Reading

References

Jul 25, 1991·Nucleic Acids Research·R H DonJ S Mattick
Feb 1, 1987·Protein Engineering·B NilssonM Uhlén
Mar 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·D BhayaA R Grossman
Nov 11, 1999·Protein Engineering·E GunneriussonP Nygren
Feb 15, 2000·Journal of Bacteriology·E Hoiczyk, A Hansel
Dec 18, 2001·Micron : the International Research and Review Journal for Microscopy·Jan SmardaVladislav Krzyzánek
Jun 22, 2002·FEMS Microbiology Letters·Henrik Wernérus, Stefan Ståhl
Oct 31, 2002·Plant & Cell Physiology·Shinobu Okamoto, Masayuki Ohmori
Dec 14, 2002·Trends in Biotechnology·Sang Yup LeeZhaohui Xu
Mar 12, 2004·Biochemical and Biophysical Research Communications·Young Hye KimYoung-Ho Chung
Nov 6, 2008·PloS One·Carola EnglerSylvestre Marillonnet
Nov 3, 2009·Nucleic Acids Research·Mitsuteru NakaoYasukazu Nakamura
Mar 27, 2010·Journal of Molecular Biology·Carmen L GiltnerLori L Burrows
Aug 24, 2010·Current Opinion in Biotechnology·Kiyotaka Shiba
Dec 7, 2010·Biochemical and Biophysical Research Communications·Young Hye KimYoung Mok Park
Feb 18, 2011·Applied Biochemistry and Biotechnology·Wipa ChungjatupornchaiSirirat Fa-Aroonsawat
Dec 24, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yu KanesakiHirofumi Yoshikawa
Feb 7, 2012·Applied and Environmental Microbiology·Daniel C DucatPamela A Silver
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Oct 17, 2012·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Danika TrautmannWolfgang R Hess
Nov 6, 2012·Biotechnology Progress·Yi Ern CheahChristie A M Peebles
Jan 23, 2013·Trends in Biotechnology·Dries VandammeKoenraad Muylaert
Sep 24, 2013·Applied and Environmental Microbiology·Josefine AnfeltElton P Hudson
Oct 1, 2013·Journal of Bacteriology·Christoph Trautner, Wim F J Vermaas
Jan 1, 2013·Computational and Structural Biotechnology Journal·Johan Nilvebrant, Sophia Hober
Oct 12, 2014·Current Opinion in Biotechnology·Philipp Savakis, Klaas J Hellingwerf

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Citations

Oct 7, 2020·Biological Chemistry·David A Russo, Julie A Z Zedler
Feb 6, 2021·Biological Chemistry·David A Russo, Julie A Z Zedler

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