Surface-displayed expression of a neutralizing epitope of ApxIIA exotoxin in Saccharomyces cerevisiae and oral administration of it for protective immune responses against challenge by Actinobacillus pleuropneumoniae

Bioscience, Biotechnology, and Biochemistry
Jung-Mi KimDae-Hyuk Kim

Abstract

A neutralizing epitope fragment of ApxIIA toxin (ApxIIA#5) of the Korean Actinobacillus pleuropneumoniae serotype 2 strain was expressed and immobilized on the cell surface of Saccharomyces cerevisiae for efficient vaccine development. Expression of ApxIIA#5 was confirmed by Western blot analysis using cell-wall proteins, and the surface display of ApxIIA#5 was further visualized under confocal microscopy. Quantitative ELISA revealed that the recombinant ApxIIA#5 directed to the cell surface consisted of approximately 16% cell-wall proteins, estimated to be 35 mg of ApxIIA#5 protein per liter of cultured cells. An immunoassay revealed that antigen-specific antibodies against ApxIIA#5 were present in the sera of mice fed recombinant ApxIIA#5-expressing yeast, but not in mice fed the wild-type nor the vector-only transformant. Moreover, the mice fed the recombinant epitope-expressing yeast were protected from injection of a lethal dose of A. pleuropneumoniae.

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Citations

Jan 26, 2013·Microbiology·Victor M Loera-MuroAlma L Guerrero-Barrera
Jul 31, 2013·Vaccine·Min-Kyoung Shin, Han Sang Yoo
Sep 23, 2014·Molecular Immunology·Heather L Wilson, Milan R Obradovic
Feb 18, 2018·Microbial Cell Factories·Jyotiranjan BalDae-Hyuk Kim
Jan 23, 2019·FEMS Yeast Research·Ravinder Kumar, Piyush Kumar

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