Surface displaying of swine IgG1 Fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction

Veterinary Research
Zehui LiuEnqi Du

Abstract

Baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. However, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application of baculovirus. A variety of approaches have been exploited to bypass the complement system in the serum. In this study, we constructed and screened a series of baculovirus vectors displaying complement interfering factors, of which a baculovirus vector displaying swine IgG1 Fc (pFc) showed the highest complement antagonism (75.6%). Flow cytometry analysis of transduced cells demonstrated that the baculovirus display of pFc had a significant increase in transduction efficiency and transgene expression of reporter genes. On this basis, a VSV-G-pseudotyped with swine IgG1 Fc surface displayed baculovirus vector was developed to express the classical swine fever virus (CSFV) E2 gene. The translational enhancers Syn21 and P10UTR were incorporated to improve the antigen expression. The E2 gene was efficiently expressed in both insect and mammalian cells. Pigs immunized with this recombinant baculovirus developed high levels of E2-specific antibody, CSFV-specific neutralizing antibod...Continue Reading

References

Sep 10, 2003·The Journal of Biological Chemistry·Christine GaboriaudGérard J Arlaud
Jul 29, 2004·Journal of Virology·Andrea FacciabeneNicola La Monica
May 7, 2005·Nature Biotechnology·Thomas A KostDonald L Jarvis
Dec 21, 2005·Immunology Letters·Andrew Getahun, Birgitta Heyman
Feb 8, 2006·The Journal of Veterinary Medical Science·Tatiana A BatanovaYasuhiro Takashima
Mar 21, 2006·Biochemical and Biophysical Research Communications·Masayuki KitajimaHiroshi Takaku
Mar 14, 2008·Combinatorial Chemistry & High Throughput Screening·Anna R Mäkelä, Christian Oker-Blom
Dec 3, 2008·Immunogenetics·J E ButlerImre Kacskovics
Aug 12, 2009·Molecular Immunology·Lindsay J GeorgopoulosBo Nilsson
Nov 13, 2009·Scandinavian Journal of Immunology·T E MichaelsenO Ihle
Aug 4, 2011·Journal of Invertebrate Pathology·Minna U KaikkonenKari J Airenne
Feb 23, 2012·Scientific Reports·David N A MekhaielRichard J Pleass
Jul 28, 2012·EMBO Molecular Medicine·Daniel M CzajkowskyRichard J Pleass
Dec 10, 2014·Trends in Biotechnology·Ditza LevinZuben E Sauna
Dec 17, 2014·Virus Research·Wei JiCheng-Qiang He
Dec 23, 2015·Vaccine·Kimia KardaniSepideh Shahbazi

❮ Previous
Next ❯

Citations

May 19, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Neda RahimianHamed Mirzaei
Jun 9, 2021·International Journal of Biological Macromolecules·Ze-Hui LiuFang He
Aug 10, 2021·Frontiers in Immunology·Ze-Hui LiuFang He

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY775178

Methods Mentioned

BETA
transgenic
IEC
PCR
flow cytometry
ELISA
fluorescence
Flow

Software Mentioned

GENEWIZ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.