Surface elastic properties of human retinal pigment epithelium melanosomes

Photochemistry and Photobiology
Senli GuoJohn D Simon

Abstract

Atomic force microscope (AFM) imaging and nanoindentation measurements in water were used to probe the mechanical properties of retinal pigment epithelium melanosomes isolated from 14-year-old and 76-year-old donors. Topographic imaging reveals surface roughness similar to previous measurements on dry melanosomes. Force-indentation measurements show different types of responses that were catalogued into four different categories. In these measurements no permanent surface damage of melanosomes was observed as revealed by imaging before and after indentation measurements. The indentation measurements that exhibited nearly elastic responses were used to determine the Young's modulus of melanosomes. The average Young's modulus values are similar for 14-year-old and 76-year-old melanosomes with a somewhat narrower distribution for the 14-year-old sample. These elastic modulus values are considerably higher than the modulus of organelles with cytoplasm (<1 MPa) and approaching values of the modulus of protein crystals (approximately 100 MPa) indicating rather high packing density of biologic material in melanosomes. The width of the Young's modulus distributions is considerable spanning from few megapascals to few tens of megapascal...Continue Reading

References

Feb 28, 1992·Journal of Photochemistry and Photobiology. B, Biology·T Sarna
Jun 1, 1971·Kybernetik·J M Findlay
Aug 1, 1994·Pigment Cell Research·J ChengP Zschack
Aug 1, 1994·Pigment Cell Research·J ChengM Eisner
Nov 1, 1996·Biophysical Journal·V Parpura, J M Fernandez
Jul 25, 1998·FEBS Letters·A Vinckier, G Semenza
Aug 24, 1999·Pigment Cell Research·U Schraermeyer, K Heimann
Jul 8, 2000·Pigment Cell Research·J B NofsingerJ D Simon
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·D SulzerL Zecca
Aug 30, 2001·Proceedings of the National Academy of Sciences of the United States of America·T KushimotoV J Hearing
Sep 6, 2001·Journal of Photochemistry and Photobiology. B, Biology·S M De LeeuwA A Schothorst
Dec 18, 2001·Journal of Photochemistry and Photobiology. B, Biology·M BoultonB Rózanowski
May 25, 2002·Pigment Cell Research·Kazumasa Wakamatsu, Shosuke Ito
Feb 19, 2003·Experimental Eye Research·Tadeusz SarnaMariusz Zareba
Mar 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicole M Haralampus-GrynaviskiJohn D Simon
Apr 15, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Taketsugu TadokoroVincent J Hearing
Aug 20, 2003·The Journal of Investigative Dermatology·Egil Kvam, Jostein Dahle
Jun 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brandon-Luke L SeagleJames R Norris
Dec 13, 2005·Free Radical Biology & Medicine·Mariusz ZarebaJanice M Burke
Apr 15, 2006·Photochemistry and Photobiology·Z WangE R Gaillard
Nov 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Brandon-Luke L SeagleJames R Norris
Nov 7, 2006·Pigment Cell Research·Paul Meredith, Tadeusz Sarna
Dec 13, 2006·Physical Review Letters·Efthimios KaxirasSheng Meng
Jun 22, 2007·The Journal of Physical Chemistry. B·Lian Hong, John D Simon
Jul 25, 2007·Photochemistry and Photobiology·Janice M BurkeTadeusz Sarna
Jan 1, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Senli Guo, Boris B Akhremitchev
Feb 12, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Alan B OelkersDavid R Tyler
Mar 11, 2008·Investigative Ophthalmology & Visual Science·Bartosz RózanowskiMałgorzata Rózanowska

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Citations

Jul 21, 2012·Cell Biochemistry and Biophysics·Augusto BrunoValeria Levi
Apr 11, 2013·Chemical Reviews·Chad P SatoriEdgar A Arriaga
Mar 14, 2014·PloS One·Thomas GorniakAxel Rosenhahn
May 8, 2013·Pigment Cell & Melanoma Research·Michal SarnaTadeusz Sarna
Jul 16, 2015·Pigment Cell & Melanoma Research·Marco d'IschiaShosuke Ito
Feb 22, 2018·International Journal of Molecular Sciences·Michal SarnaKrystyna Urbanska
Sep 3, 2014·Experimental Dermatology·Michal SarnaTadeusz Sarna
Sep 4, 2018·Frontiers in Bioengineering and Biotechnology·Vincent Ball
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Li SunAiguo Wu
Jun 28, 2019·Scientific Reports·Michal SarnaKrystyna Urbanska
Jul 22, 2020·Physical Review. E·A B Fernández CasafuzL Bruno
Sep 4, 2020·Vestnik oftalmologii·A A GamidovZ V Surnina
Feb 9, 2021·Global Challenges·Hanaa A GalebJohn G Hardy
Sep 27, 2008·The Journal of Physical Chemistry. B·John D SimonDana N Peles

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