Surface enhanced laser desorption ionization spectrometry reveals biomarkers for drug treatment but not dose

Proteomics
Cloud P PaweletzRonald C Hendrickson

Abstract

Here we describe the use of SELDI-MS to detect dose-dependent peptide changes in plasma from mice treated with vehicle or rosiglitazone at one of two doses (10 and 30 mg/kg). SELDI features differentiating spectra from the three conditions were found and used to train classifiers. Samples treated with vehicle could be reliably distinguished from samples treated with either dose, but samples treated with the different doses could not be reliably distinguished from one another. We conclude that while SELDI-TOF mass spectra can be used to distinguish treated from untreated samples, the reproducibility and information content of SELDI-TOF are currently not sufficient as a pharmacodynamic readout to distinguish between mice treated with 10 or 30 mg/kg of rosiglitazone. This raises more general questions about whether SELDI's sensitivity is sufficient for detecting dose-dependent changes in plasma.

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Citations

Apr 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Matthew T MazurRonald C Hendrickson
Sep 26, 2007·Expert Review of Molecular Diagnostics·Harald TammenHans-Dieter Zucht
Oct 6, 2007·Expert Opinion on Drug Metabolism & Toxicology·Ben C CollinsStephen R Pennington
Dec 5, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Supawadee SriyamShui-Tein Chen
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Nov 9, 2010·Biomedical Chromatography : BMC·Ching-Seng AngEdouard C Nice

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