Surface-Enhanced Raman Scattering Based Microfluidics for Single-Cell Analysis

Analytical Chemistry
Marjorie R WillnerMichele Zagnoni

Abstract

The integration of surface-enhanced Raman scattering (SERS) with droplet microfluidics has the potential to improve our understanding of cellular systems. Herein, we present the first application of SERS droplet microfluidics for single-cell analysis. A microfluidic device was used to encapsulate single prostate cancer cells and wheat germ agglutin (WGA)-functionalized SERS nanoprobes in water-in-oil droplets that were subsequently locked into a storage droplet array for spectroscopic investigation. The stationary droplets enabled the rapid identification of SERS regions of interest in live cancer cells by allowing collection of "fast" coarse maps over an area of several square millimeters followed by "slower" detailed interrogation of the identified hotspots. We demonstrate SERS at cellular resolution via a proof-of-concept assay that detects glycan expression on the surface of prostate cancer cells using WGA-modified metallic nanoparticles. The data illustrates the potential of SERS optofluidic systems for high-throughput cell screening and illustrates a previously unobserved high degree of cell-to-cell variability in the size and number of glycan islands.

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Citations

May 31, 2019·Cancers·Luca Guerrini, Ramon A Alvarez-Puebla
Jul 3, 2020·The Journal of Chemical Physics·Loza F TadesseJennifer Dionne
Aug 7, 2020·Nanoscale Horizons : the Home for Rapid Reports of Exceptional Significance in Nanoscience and Nanotechnolgy·Lucio LittiLuis M Liz-Marzán
Jan 23, 2021·Applied Microbiology and Biotechnology·Shuaishuai YanQing Liu
Feb 26, 2021·Journal of Separation Science·Ling Xia, Gongke Li
Jul 13, 2021·Critical Reviews in Food Science and Nutrition·Heera JayanDa-Wen Sun
Aug 3, 2021·Trends in Analytical Chemistry : TRAC·Asifur RahmanPeter J Vikesland
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich
Sep 11, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaojia LiuXing Ma

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