Surface-enhanced Raman scattering reveals adsorption of mitoxantrone on plasma membrane of living cells

Biochemical and Biophysical Research Communications
G BreuzardJ-M Millot

Abstract

Surface-enhanced Raman scattering (SERS) spectroscopy was applied to analyze mitoxantrone (MTX) adsorption on the plasma membrane microenvironment of sensitive (HCT-116 S) or BCRP/MXR-type resistant (HCT-116 R) cells. The addition of silver colloid to MTX-treated cells revealed an enhanced Raman scattering of MTX. Addition of extracellular DNA induced a total extinction of MTX Raman intensity for both cell lines, which revealed an adsorption of MTX on plasma membrane. A threefold higher MTX Raman intensity was observed for HCT-116 R, suggesting a tight MTX adsorption in the plasma membrane microenvironment. Fluorescence confocal microscopy confirmed a relative MTX emission around plasma membrane for HCT-116 R. After 30 min at 4 degrees C, a threefold decrease of the MTX Raman scattering was observed for HCT-116 R, contrary to HCT-116 S. Permeation with benzyl alcohol revealed a threefold decrease of membrane MTX adsorption on HCT-116 R, exclusively. This additional MTX adsorption should correspond to the drug bound to an unstable site on the HCT-116 R membrane. This study showed that SERS spectroscopy could be a direct method to reveal drug adsorption to the membrane environment of living cells.

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Apr 30, 2008·Chemical Society Reviews·Igor ChourpaGanesh D Sockalingum
Jan 13, 2006·Annual Review of Pharmacology and Toxicology·P Krishnamurthy, J D Schuetz
Feb 28, 2013·Chemistry Central Journal·Jichun ZhuZhenrong Sun
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Feb 22, 2005·Biochemical and Biophysical Research Communications·G BreuzardJ-M Millot
Aug 2, 2005·Current Opinion in Chemical Biology·Alan G Ryder
Apr 17, 2007·Journal of Photochemistry and Photobiology. B, Biology·Gilles BreuzardJean-Marc Millot
Jul 12, 2014·Applied Spectroscopy·Sevda Mert, Mustafa Çulha
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Oct 15, 2011·Analytica Chimica Acta·Eleonora Petryayeva, Ulrich J Krull

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