Surface-enhanced Raman spectroscopic detection of a bacteria biomarker using gold nanoparticle immobilized substrates

Analytical Chemistry
Han-Wen ChengRu-Qin Yu

Abstract

The development of ultrasensitive and rapid methods for the detection of dipicolinic acid (DPA), a biomarker for bacterial spores including Bacillus anthracis, is increasingly important. This paper reports the results of an investigation of surface enhanced Raman spectroscopy (SERS) based ultrasensitive detection of DPA using a gold nanoparticle/polyvinylpyrrolidone/gold substrate (AuNPs/PVP/Au). The strong SERS effect of this substrate exploits the particle-particle and particle-substrate plasmonic coupling, which is optimized by manipulating the diameter of the nanoparticles (50-70 nm). The correlation between the SERS intensity of the diagnostic band and the DPA concentration (0.1 ppb to 100 ppm) was shown to exhibit two linear regions, i.e., the low- (<0.01 ppm) and high-concentration (>1 ppm) regions, with an intermediate region in between. The presence of a linear relationship in the low-concentration region was observed for the first time in SERS detection of DPA. A detection limit of 0.1 ppb was obtained from the substrates with 60 nm sized Au NPs, which is, to our knowledge, the lowest detection limit reported for DPA using this type of SERS substrate. This finding was also supported by the estimated enhancement factor...Continue Reading

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Citations

Feb 23, 2013·Biosensors & Bioelectronics·Jing ChenBiswajit Das
Mar 28, 2018·Advanced Healthcare Materials·Peiyan YuanQing-Hua Xu
Apr 25, 2012·Chemistry : a European Journal·Mirja HartmannAnke Krueger
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Aug 12, 2014·ACS Applied Materials & Interfaces·Zakiya SkeeteChuan-Jian Zhong
Aug 20, 2021·The Journal of Physical Chemistry Letters·Swati TanwarTapasi Sen
Nov 14, 2017·Journal of the American Chemical Society·Swati TanwarTapasi Sen
Nov 30, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Mengmeng ChenYan Fang
Aug 4, 2020·ACS Applied Nano Materials·Paulo De Carvalho GomesPola Goldberg Oppenheimer

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