Surface-enhanced Raman spectroscopy and MCR-ALS for the selective sensing of urinary adenosine on filter paper

Talanta
Javier E L VillaRonei J Poppi

Abstract

Adenosine is a purine nucleoside that is present in all human cells and is essential for regulating certain physiological activities in tissues and organs. Since adenosine is considered to be a potential cancer biomarker in urine, its determination may be crucial for the early diagnosis and non-invasive monitoring of cancer. Herein, we present a label-free method to quantify urinary adenosine using surface-enhanced Raman spectroscopy (SERS) and multivariate curve resolution-alternating least squares (MCR-ALS). Ring-oven preconcentration and direct deposition of monodisperse gold nanoparticles on filter paper were employed to improve the sampling efficiency. Further, MCR-ALS (assessed with and without a correlation constraint), the standard addition method and pH controls were combined to compensate for the matrix effect and to address overlapping bands in the analysis of human urine samples. As a result, the proposed method showed to be sensitive (LOD varying between 3.8 and 4.9 µmol L-1, S/R = 3), reproducible (RSD less than ± 15%), and selective over other nucleosides (guanosine, cytidine, thymidine and uridine) and unknown interferences (second-order advantage). This is the first report of a SERS-chemometric method applied t...Continue Reading

Citations

Nov 18, 2020·Analytical Methods : Advancing Methods and Applications·Dan LiuGuohua Zhang
Dec 8, 2020·Biomedical Optics Express·Wei ZhangAnhong Zhou
May 4, 2021·Talanta·Maria AntónioAna L Daniel-da-Silva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.