Surface-enhanced Raman spectroscopy for bacterial discrimination utilizing a scanning electron microscope with a Raman spectroscopy interface

Analytical Chemistry
Roger M JarvisRoyston Goodacre

Abstract

Surface-enhanced Raman scattering (SERS) utilizing colloidal silver has already been shown to provide a rapid means of generating "whole-organism fingerprints" for use in bacterial identification and discrimination. However, one of the main drawbacks of the technique for the analysis of microbiological samples with optical Raman microspectroscopy has been the inability to acquire pre-emptively a region of the sample matrix where both the SERS substrate and biomass are both present. In this study, we introduce a Raman interface for scanning electron microscopy (SEM) and demonstrate the application of this technology to the reproducible and targeted collection of bacterial SERS spectra. In secondary electron mode, the SEM images clearly reveal regions of the sample matrix where the sodium borohydride-reduced silver colloidal particles are present, Stokes spectra collected from these regions are rich in vibrational bands, whereas spectra taken from other areas of the sample elicit a strong fluorescence response. Replicate SERS spectra were collected from two bacterial strains and show excellent reproducibility both by visual inspection and as demonstrated by principal components analysis on the whole SERS spectra.

References

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Feb 12, 2002·Journal of the American Society for Mass Spectrometry·Seetharaman VaidyanathanRoyston Goodacre
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Citations

Aug 26, 2006·Analytical and Bioanalytical Chemistry·Atanu SenguptaE James Davis
Nov 15, 2007·Analytical and Bioanalytical Chemistry·Katharina HeringJürgen Popp
May 2, 2009·Analytical and Bioanalytical Chemistry·P R Stoddart, D J White
Apr 20, 2010·Analytical and Bioanalytical Chemistry·Erhan TemurNihal Aydogan
Dec 8, 2010·Analytical and Bioanalytical Chemistry·Yuling WangJoseph Irudayaraj
Feb 23, 2013·Biosensors & Bioelectronics·Jing ChenBiswajit Das
Apr 30, 2008·Chemical Society Reviews·Elena Bailo, Volker Deckert
Apr 30, 2008·Chemical Society Reviews·Roger M Jarvis, Royston Goodacre
Mar 8, 2011·Chemical Communications : Chem Comm·Sandra Preciado-FloresJin Z Zhang
Nov 3, 2010·Applied and Environmental Microbiology·Sunil PalchaudhuriJames Stenger
Jul 14, 2014·Micron : the International Research and Review Journal for Microscopy·Guillaume WilleAbdeltif Lahfid
Nov 13, 2007·Journal of Biomedical Optics·Mehmet KahramanMustafa Culha
Jan 12, 2016·Analytical Chemistry·Dillon SchiffYaakov R Tischler
Mar 15, 2016·Analytical and Bioanalytical Chemistry·Christoph Haisch
Apr 22, 2016·Analytical and Bioanalytical Chemistry·W Ranjith PremasiriLawrence D Ziegler
Jul 19, 2016·Analytical Chemistry·Anna K BoardmanAlexis F Sauer-Budge
Jul 13, 2006·Faraday Discussions·Roger M JarvisRoyston Goodacre
Oct 8, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Li CuiKaisong Zhang
Nov 7, 2009·Applied Spectroscopy·Ismail SayinMustafa Culha
Nov 15, 2008·Applied Spectroscopy·Mustafa CulhaMedine Güllüce
Apr 23, 2013·Applied Spectroscopy·Jason A GuicheteauDarren Emge
Mar 28, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Farzaneh MoghtaderErhan Piskin
Feb 5, 2016·The Analyst·Christopher J PulliamR Graham Cooks
Sep 8, 2017·Faraday Discussions·Malama ChisangaRoyston Goodacre

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