Surface-enhanced Raman spectroscopy for detection of toxic amyloid β oligomers adsorbed on self-assembled monolayers

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Vladislava VoiciukGediminas Niaura

Abstract

Surface-enhanced Raman spectroscopy (SERS) was used to detect different spectral features of small (1-2 nm) and large (5-10 nm) synthetic amyloid Aβ-42 oligomers, exhibiting high and no detectable neurotoxicities, respectively. Adsorption of peptides at self-assembled monolayers (SAM) terminated by methyl and pyridinium groups was employed to differentiate toxic and non-toxic oligomers. Three SAMs were analyzed: hydrophobic heptanethiol (HT) and octadecanethiol (ODT) as well as positively charged N-(6-mercapto)hexylpyridinium (MHP) chloride. SERS study revealed twofold adsorption effect, changes in the monolayer structure and appearance of new bands associated with the adsorbed peptides. A band at 1387 cm(-1), observed as a result of the SAM and Aβ-42 interaction, is tentatively assigned to the peptide symmetric stretching vibration of carboxylate groups, and appears to be the most prominent spectral feature distinguishing toxic oligomers from the non-toxic Aβ-42 forms. This band was identified in the spectra of Aβ-42 adsorption on heptanethiol and MHP monolayers, while no clear perturbations were observed in the case of ODT monolayer.

References

Jul 28, 2004·Langmuir : the ACS Journal of Surfaces and Colloids·G ValinciusV Razumas
Jun 24, 2008·Critical Reviews in Food Science and Nutrition·Ana M Herrero
Dec 2, 2008·Journal of Colloid and Interface Science·Rubens A SilvaDenise F S Petri
May 18, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·A E AliagaS Sanchez-Cortes

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Citations

Mar 21, 2016·Chemistry, an Asian Journal·Yanli ZhouMaotian Xu
Apr 28, 2018·Protein Science : a Publication of the Protein Society·Xinke YuYa-Hong Xie
Feb 6, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Caterina CrediFrancesco Saverio Pavone
Jan 9, 2018·ACS Chemical Neuroscience·George DevittSumeet Mahajan

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