Surface-enhanced Raman spectroscopy for in situ measurements of signaling molecules (autoinducers) relevant to bacteria quorum sensing

Applied Spectroscopy
William F PearmanAlan W Decho

Abstract

Autoinducer (AI) molecules are used by quorum sensing (QS) bacteria to communicate information about their environment and are critical to their ability to coordinate certain physiological activities. Studying how these organisms react to environmental stresses could provide insight into methods to control these activities. To this end, we are investigating spectroscopic methods of analysis that allow in situ measurements of these AI molecules under different environmental conditions. We found that for one class of AIs, N-acyl-homoserine lactones (AHLs), surface-enhanced Raman spectroscopy (SERS) is a method capable of performing such measurements in situ. SERS spectra of seven different AHLs with acyl chain lengths from 4 to 12 carbons were collected for the first time using Ag colloidal nanoparticles synthesized via both citrate and borohydride reduction methods. Strong SERS spectra were obtained in as little as 10 seconds for 80 microM solutions of AI that exhibited the strongest SERS response, whereas 20 seconds was typical for most AI SERS spectra collected during this study. Although all spectra were similar, significant differences were detected in the SERS spectra of C4-AHL and 3-oxo-C6-AHL and more subtle differences w...Continue Reading

References

Aug 19, 2000·Infection and Immunity·T R de Kievit, B H Iglewski
Feb 7, 2001·Applied and Environmental Microbiology·J B AndersenM Givskov
Aug 29, 2001·FEMS Microbiology Reviews·N A WhiteheadG P Salmond
Sep 7, 2001·Annual Review of Microbiology·M B Miller, B L Bassler
Dec 26, 2001·Chemical Reviews·K KneippM S Feld
Aug 6, 2002·Trends in Microbiology·Rosemary J Redfield
Aug 16, 2002·Current Opinion in Plant Biology·John LohArun Chatterjee
Sep 5, 2002·Nature Reviews. Molecular Cell Biology·Clay Fuqua, E Peter Greenberg
Jan 14, 2003·Journal of Biotechnology·Feng XuHeinz-Josef Dussen
Jan 22, 2004·The Journal of Biological Chemistry·Lian-Hui WangLian-Hui Zhang
Jul 13, 2006·Faraday Discussions·Roger M JarvisRoyston Goodacre
Aug 26, 2006·Analytical and Bioanalytical Chemistry·Atanu SenguptaE James Davis
Sep 28, 2006·Applied Spectroscopy·Maria Fernanda EscorizaJohn Maier

❮ Previous
Next ❯

Citations

Jul 16, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Maria KnauerChristoph Haisch
Oct 22, 2008·Applied Spectroscopy·William F PearmanSherwood Hall
May 7, 2013·Applied Spectroscopy·Esen Efeoglu, Mustafa Culha
May 31, 2020·International Journal of Molecular Sciences·Barbara GierobaAnna Sroka-Bartnicka
Apr 28, 2017·Frontiers in Neuroscience·Frederick VerbekeBart De Spiegeleer
Jun 6, 2018·Frontiers in Cellular and Infection Microbiology·Gustavo BodelónIsabel Pastoriza-Santos
Aug 29, 2018·Nanomaterials·Tomasz SzymborskiAgnieszka Kamińska
Jan 9, 2010·Inorganic Chemistry·Dawn WallaceW Ewen Smith

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.